C7955 Molecular luminescence

Faculty of Science
Autumn 2010
Extent and Intensity
1/0/0. (plus 2 credits for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Petr Táborský, Ph.D. (lecturer)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jan Preisler, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Wed 16:00–16:50 C14/207
Prerequisites
Knowledge of analytical chemistry on a level of introductory course. Knowledge of high school physics.
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30
Course objectives
The course will provide information on physical principles of molecular luminescence and its applications in inorganic, organic and especially biochemical analysis. At the end of the course students also should understand to modern application of luminescence spectroscopy and related techniques such as GFP, FRET, FLIM, polarisation of fluorescence, Fluorescence in situ hybridization, etc.
Syllabus
  • 1. Introduction to optical methods, history. Absorption of light by molecule, electron transitions, Franck-Condon principle. Jablonskis diagram, luminescence, fluorescence, phosphorescence, delayed fluorescence, basic principles. Influence of medium, pH, polarity, etc. Luminescence quenching, dynamic and static collissions. 2. Basic units, luminescence spectra: excitation and emission spectra, Stokes shift, mirror rule. Scattering. Basic relationships, energetic and quantum yields. Luminescence lifetime. 3. Strucutre of compounds and luminescence, luminescence of organic molecules, transitions, general rules. Fluorescence spectra of biomolecules. Luminescence properties of inorganic compounds. Luminescence of solid phase. Interpretation of spectra: concentration, structure. Intrinsic and extrinsic luminescence. 4. Instrumentation, basic system, spectrofluorometers, excitation sources, monochromators and detectors. 5. Measurement and data treatment, 3D spectra, synchronous scan. Lifetime measurement. Fluorescence microscopy. Phosphorescence measurement. Palarisation and luminescence anisotropy. 6. Luminescence assignment: chemiluminescence, bioluminescence, elektroluminescence, triboluminescence, etc. 7. Luminescence labels, derivatisation, binding sites, criterion, luminescence in clinical analysis, fluorescence imunoassays,fluor. substrates. 8. Comparision to spectrophotometry in UV-Vis region. Separation techniques with fluorescence detection, indirect determination. 9. Luminescence probes: polarity, membarne and DNA probes, sensors. 10. Fluorescence microscopy and time resolved fluorescence microscopy, flow cytometry. FISH. Fluorescence correlation spectroscopy. 11. Native luminescence of proteins and peptides, membrane modifications, Green fluorescence protein. 12. Excurssion
Literature
  • Principles of fluorescence spectroscopy. Edited by Joseph R. Lakowicz. 3rd ed. New York: Springer, 2006, xxvi, 954. ISBN 0387312781. info
Teaching methods
lectures
Assessment methods
Final oral examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Teacher's information
http://bart.chemi.muni.cz/courses.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2010, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2010/C7955