IB025 Status, issues and perspectives of protein complexes isolation from plant cells

Faculty of Science
Autumn 2010
Extent and Intensity
0/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. Geert de Jaeger (lecturer)
Guaranteed by
doc. RNDr. Jan Hejátko, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Defining protein complexes is critical to virtually all aspects of cell biology because most cellular processes are regulated by stable or more dynamic protein interactions. So, elucidation of the protein protein interaction network around proteins is essential to fully understand their function and regulation. In the last decade, new technologies emerged to study protein protein interactions under near physiological conditions. We have developed a high throughput tandem affinity purification (TAP)/mass spectrometry (MS) platform to analyze protein complexes in Arabidopsis thaliana (1, 2). This streamlined platform follows an integrated approach comprising generic Gateway based vectors with high cloning flexibility, the fast generation of transgenic biomass, TAP adapted for plant cells, and tandem matrix assisted laser desorption ionization-MS for the identification of purified proteins. Our technology will be presented in mere detail based on a series of concrete experimental results. Moreover, technological hurdles and futur approaches will be discussed.
Language of instruction
English
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught only once.
The course is taught in blocks.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2011 - acreditation.
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