Bi7430c Molecular biotechnology - practical course

Faculty of Science
Autumn 2013
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
Koen Beerens, PhD. (seminar tutor)
Mgr. Lukáš Chrást (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Mgr. Veronika Štěpánková, Ph.D. (seminar tutor)
Mgr. Hana Moskalíková (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 14:00–15:50 C03/208
Prerequisites
NOW ( Bi7430 Molecular biotechnology )
Parallel attendance of Bi7430 Molecular Biotechnology lecture.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will have practical experience with range of procedures of molecular biotechnology methods, e.g. design of primers, DNA cleavage using restrictive enzymes, bacterial cell transformation, fermentation cultivation of E. coli, recombinant protein purification and its use in biocatalysis and biosensing.
Syllabus
  • BLOK A
  • 1. Preparation and transformation of liposoms
  • (preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer)
  • 2. Analysis of liposoms
  • (Single particle tracking analysis by using Nanosight 500, transmission electron microscopy)
  • BLOK B
  • 3. Preparation of recombinant E. coli
  • (Clone Manager, design of primers, restriction cleavage, cloning, transformation)
  • 4. Cultivation of recombinant E. coli
  • (fermentation of E. coli biomass and expression of recombinant protein)
  • 5. Purification of recombinant protein
  • (disintegration of biomass, purification of recombinant enzyme, gel electrophoresis)
  • 6. Preparation of biosensor
  • (co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte)
  • 7. Biocatalytic preparation of pharmaceutical precursor
  • (biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme)
Literature
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell. xxxiii, 60. ISBN 9783527326372. 2011. info
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press. xvi, 1000. ISBN 9781555814984. 2010. info
Teaching methods
In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample. The results of experiments are evaluated by each student in separate protocols (according to the outline: introduction, aim, material and methods, results, discussion, conclusion). Each student describes analyses, evaluates and discusses the results individually and independently.
Assessment methods
Students will obtain positive assessment of the practice for active participation in exercises and preparation of protocols from each exercise.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Course is no more offered.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2013, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2013/Bi7430c