S1002 Chemical properties, structure and interactions of nucleic acids – practical course

Faculty of Science
Autumn 2014
Extent and Intensity
0/10. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
doc. RNDr. Miroslav Fojta, CSc. (seminar tutor)
prof. RNDr. Michaela Vorlíčková, DrSc. (seminar tutor)
doc. Mgr. Miloslava Fojtová, CSc. (seminar tutor)
RNDr. Iva Kejnovská, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Miroslav Fojta, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Miroslav Fojta, CSc.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
Previous or parallel passing-out the S1001 subject
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
At the end of the course students will be trained to perform simple experiments based on methods and experimental techniques that are related to topics of the course S1001.
Syllabus
  • 1. Gel electrophoresis of nucleic acids, proteins and their complexes, two-dimesional electrophoresis. 2. Blotting, protein immunodetection. 3. Magnatoseparation techniques, immunoprecipitation. 4. Centrifugation methods, DNA precipitation, extraction with organic solvents, gradient centrifugation, ultrafiltration. 5. Chromatographic methods, FPLC of proteins on ionex, affinity and gel-filtration columns. 7. DNA sequencing and footprinting. 7. Electrochemical methods, determination of DNA and proteins, detection of DNA damage and interactions. 8. UV VIS and CD spectrophotometry of biopolymers.
Literature
  • BLACKBURN, M. G. and M. J. GAIT. Nucleic Acids in Chemistry and Biology. Oxford: Oxford University Press, 1996. info
  • SINDEN, Richard R. DNA structure and function. San Diego: Academic Press, 1994, xxiii, 398. ISBN 0126457506. info
  • ADAMS, R. L. P., J. T. KNOWLER and D. P. LEADER. The Biochemistry of Nucleic Acids. 10th edit. London: Chapman and Hall, 1986. info
Teaching methods
Practical courses, demonstrations in the laboratory
Assessment methods
Teaching method: practical demonstrations of instruments and real experimental data, simple experimental tasks conducted by the students Type of exam: credit (given on the basis of participance)
Language of instruction
English
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Autumn 2011 - acreditation, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2014, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2014/S1002