PřF:Bi6700c Taxonomy of prok.-lab.course - Course Information
Bi6700c Taxonomy of prok.-lab.course
Faculty of ScienceAutumn 2016
- Extent and Intensity
- 0/4/0. 4 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Ivo Sedláček, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Ivo Sedláček, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Ivo Sedláček, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- NOW( Bi6700 Taxonomy of prokaryotes )
Examination - basic microbiology, physiology of bacteria, biochemistry, molecular biology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, B-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, B-EXB)
- Special Biology (programme PřF, B-EXB, specialization Mikrobiology a Molecular Biotechnology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course students should be able to perform: - isolation and cultivation of microorganisms, maintenance of pure cultures; - microscopic and macroscopic morphology of archaea and bacteria; - phenotypic classification and identification of Gram negative bacteria; - phenotypic classification and identification of Gram positive bacteria; - typing and identification of bacteria by rep-PCR method; - analysis of microorganisms by mass spectrometry MALDI TOF procedure.
- Syllabus
- Agar and broth media
- Isolation
- Cultivation
- Morphology
- Classification of fermenting rods
- Classification of non-fermenting rods
- Classification of G+ k cocci and rods (phylum Actinobacteria, Firmicutes)
- rep_PCR
- MALDI TOF MS
- Evaluation of identification
- Literature
- SEDLÁČEK, Ivo. Taxonomie prokaryot (Taxonomy of prokaryotes). 1st ed. Brno: Masarykova univerzita, 2007, 270 pp. 55-969C-2006 02/58 12Př. ISBN 80-210-4207-9. info
- Goodfellow M. and O Donnell A.G. : Chemical methoda in procaryotic systematics. J.Wiley and sons, N.Y., 1994
- Gerhardt, P., Murray, R.G.E., Wood, W.A., Krieg, N.R. (eds.). 1994. Methods for general and molecular bacteriology. ASM, Washington, D.C.
- Murray, PB., Baron, E.J., Jorgensen, J.H., Pfaller, M.A., Yolken, R.H. (eds.). 2003. Manual of clinical microbioloogy. Volume 1. 8th edition. ASM Press. Washington, D.C.
- Pershing, D.H., Tenover, F.C., Versalovic, J., Tang, Y.W., Unger, E.R., Relman, D.A., White, TJ. (eds.). 2004. Molecular microbiology: diagnostic principles and practice. ASM Press, Washington, USA.
- Teaching methods
- Individual study followed by laboratory practice.
- Assessment methods
- Two written tests before course (30 and 50 questions), lacoratory courses, credit (course).
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Limitujici je kapacita cvicebny
- Enrolment Statistics (Autumn 2016, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2016/Bi6700c