PřF:C7895 Mass Spectrometry - Course Information
C7895 Mass Spectrometry of BiomoleculesFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- prof. Mgr. Jan Preisler, Ph.D. (lecturer)
Mgr. Iva Benešová, Ph.D. (assistant)
- Guaranteed by
- prof. Mgr. Jan Preisler, Ph.D.
Department of Chemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Chemistry - Chemistry Section - Faculty of Science
- Mon 17. 9. to Fri 14. 12. Wed 13:00–14:50 C14/207
- Knowledge of Analytical Chemistry on a level of introductory course Knowledge of high school Physics. The course is given in Czech in odd years and in English in even years or as requested by students. The final oral examination will be in Czech or English.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Course objectives
- Student will understand fundamentals of mass spektrometry including ionization methods, mass analyzers, ion detectors and selected applications, e.g. ionization mtechniques MALDI and ESI, modern mass snalyzers time-of-flight, ion traps and orbitrap, identification and quantification of proteins.
- 1. A Brief Historical Perspective: Overview of Techniques and Technology. Basic Concepts of MS (resolution, sensitivity).
- 2. Ionization Techniques and Sample Introduction: Electron Impact (EI). Chemical Ionization (CI). Glow Discharge. Inductively Coupled Plasma (ICP). Fast Atom Bombardment (FAB). Secondary Ion Mass Spectrometry (SIMS). Thermospray (TSI). Electrospray (ESI). Laser Desorption (LD). Plasma Desorption (PD). Matrix-Assisted Laser Desorption/Ionization (MALDI). Coupling of Separation and MS (on-line, off-line, chips)
- 3. Mass Spectrometers: Ion Optics. Simulation of Ion Movement (Simion). Energy Analyzers. Magnetic Sector. Quadrupole Mass Analyzer. Ion Cyclotron Resonance Fourier Transform Mass Spectrometer (FT-ICR-MS). Ion Trap (IT). Linear Trap (LT). Orbitrap. Time-of-Flight Mass Spectrometer (TOFMS). Collision-Induced Dissociation (CID). Tandem Mass Spectrometry (MS/MS). Vacuum Principles & Techniques. Detectors & Detection Electronics.
- 4. MS Applications: Proteins and peptides. Peptide mapping, protein databases. DNA. Saccharides. Synthetic polymers.
- recommended literature
- COTTER, Robert J. Time-of-Flight Mass Spectrometry: Instrumentation and applications in biological research. Washington, D.C.: American Chemical Society, 1997. 326 pp. ISBN 0-8412-3474-4. info
- COLE, Richard B. Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation & Applications. John Wiley & Sons, Inc., 1997. 577 pp. ISBN 0-471-14564-5. info
- Teaching methods
- Lectures and discussion in the end of the semester. Visit of a mass spectrometry laboratory, demonstrations of MS hardware.
- Assessment methods
- Final oral examination (in Czech or English). The exam consists of three questions on the following topics: 1. ionization techniques, 2. mass analyzers, 3. applications. Fifty percent of correct answers is needed to pass.
- Language of instruction
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: V podzimním semestru 2011 bude předmět vyučován v anglickém jazyce. Zkoušku je možné složit v češtině.
- Teacher's information