PřF:S2002 Methods plant cytogenetics 1 - Course Information
S2002 Methods in plant cytogenetics and cytogenomics I. - practical courseFaculty of Science
- Extent and Intensity
- 0/24. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
- RNDr. Terezie Mandáková, Ph.D. (seminar tutor)
- doc. Mgr. Martin Lysák, Ph.D., DSc.
National Centre for Biomolecular Research - Faculty of Science
Contact Person: RNDr. Terezie Mandáková, Ph.D.
Supplier department: National Centre for Biomolecular Research - Faculty of Science
- Course Enrolment Limitations
- The course is offered to students of any study field.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 5/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
- Annotation This practical course will demonstrate classical as well as state-of-the-art cytogenetic techniques. The accent is put on cytogenetic methods beneficial to taxonomic, biosystematic and phylogeographic studies. Students will get acquainted with preparation of mitotic and meiotic chromosomes, chromosome counting, the course of male meiosis as well as with principles of light and fluorescence microscopy. Molecular cytogenetic techniques will include DNA probe labelling, fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and chromosome painting. Digital processing of captured chromosome images will be demonstrated. Syllabus Day 1 - introduction - preparation of mitotic chromosomes from root tip meristems (Nicotiana, Festuca x Lolium hybrids) and preparation of meiotic chromosome spreads from anthers - theoretical and practical principles of light and fluorescence microscopy - analysis of chromosome preparations using fluorescence microscopy (selection of suitable slides for Day 2) - direct and indirect labelling of DNA probes (amplification of the Arabidopsis-type telomere repeat by PCR; labelling of ribosomal DNA repeats, chromosome-specific BAC clones, and genomic DNA using nick translation) Day 2 - introduction - fluorescence in situ hybridization (FISH), chromosome painting, genomic in situ hybridization (GISH): PART I (slide pre-treatment, preparation of hybridization mix, probe denaturation) - stages of mitosis and meiosis (movie, meiosis analysed using phase and fluorescence microscopy, digital microscope photography) Day 3 - introduction - FISH, chromosome painting, GISH: PART II (fluorescence detection of hybridized DNA probes) - fluorescence microscopy, image capture - computer processing of microscope images (Adobe Photoshop) - conclusions
- Language of instruction
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
- Listed among pre-requisites of other courses