PřF:C7301 Genomics - practice - Course Information

C7301 Genomics - practice

Extent and Intensity

0/0/3. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).

Teacher(s)

doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Ing. Eliška Kadlecová (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
RNDr. Jan Skalák, Ph.D. (seminar tutor)
doc. Mgr. Markéta Šámalová, Ph.D. (seminar tutor)
Mgr. Markéta Žďárská, Ph.D. (seminar tutor)

Guaranteed by

doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Prerequisites

NOW( C7201 Genomics ) || NOW( CG020 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

At the end of the course, students will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment.

Learning outcomes

At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.

Syllabus

• Design of oligonucleotides sequence
• Oligonucleotides synthesis
• Analysis of gene expression using transcriptional fusion
• Isolation of plant genomic DNA
• Identificatio of insertional mutant using PCR
• Working with databases of molecular-biology information
• Transfer of DNA on membrane, preparation of labeled probe and hybridization
• Detection of hybridized probe
• Fragmentation analysis

Literature

• Functional genomics : methods and protocols. Edited by Michael J. Brownstein - Arkady B. Khodursky. Totowa, N.J.: Humana Press, 2003, xii, 258. ISBN 1588292916. info
• Plant functional genomics. Edited by Erich Grotewold. Totowa, N.J.: Humana Press, 2003, xv, 449 s. ISBN 1588291456. info

Teaching methods

Practical solution of problems demonstrating utilizing of basic instruments of the functional genomics.

Assessment methods

The course will end up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.

Language of instruction

Czech

Follow-Up Courses

• CG020 Genomics
• C7201 Genomics - a basic course

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201

• Enrolment Statistics (autumn 2021, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2021/C7301