PřF:CB070 Protein crystallography - Course Information
CB070 Protein crystallography
Faculty of ScienceAutumn 2023
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer) - Guaranteed by
- doc. RNDr. Jaromír Marek, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Tue 12:00–12:50 C04/211
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Analytical Chemistry (programme PřF, D-CH) (2)
- Inorganic Chemistry (programme PřF, D-CH) (2)
- Physical Chemistry (programme PřF, D-CH) (2)
- Macromolecular Chemistry (programme PřF, D-CH) (2)
- Environmental Chemistry (programme PřF, D-CH) (2)
- Organic Chemistry (programme PřF, D-CH) (2)
- Course objectives
- The aim of the course is to explain to students the main principles of single crystal structural analysis, the theoretical background of diffraction on single crystal, the technical equipment used during diffraction experiments and the basic steps and methods used during processing of experimental data and solving of protein structures. The course CB070 is closely connected to exercise CB080.
- Learning outcomes
- At the end of this course, student will be: - able to understand and explain basic principles of monocrystal x-ray crystallography - introduced to history of x-ray crystallography - introduced to x-ray crystallography instrumentation - introduced to basic methodology of x-ray crystallography - introduced to practical examples from the fiel
- Syllabus
- Symmetry of matter. Interaction of X-ray with matter
- Diffraction by crystal
- Sources and detectors of X-ray. Diffractometer
- Phase problem
- Patterson and direct methods
- Refinement, R-factors, Least-square-method
- Programs SHELXS and SHELXL, CNS and CCP4.
- Crystallisation of protein crystals
- Methods of heavy derivatives of proteins
- Molecular replacement
- Refinement of protein structures
- Crystallographic databases.
- Literature
- MAREK, Jaromír and Z. TRÁVNÍČEK. Monokrystalová rentgenová strukturní analýza (Single crystal X-ray structure analysis). první. Olomouc: Vydavatelství Univerzity Palackého, 2002, 169 pp. nedělí se na edice. ISBN 80-244-0551-2. info
- GIACOVAZZO, C. Fundamentals of Crystallography. 1992. ISBN 0-19-855578-4. info
- Teaching methods
- Lectures. Home exercises at PC.
- Assessment methods
- Oral exam. Home exercises at PC.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Autumn 2023, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2023/CB070