PřF:B6270p Cytogenetics - Course Information

B6270p Cytogenetics

Faculty of Science
Spring 2000

Extent and Intensity

2/0/0. 0 credit(s). Type of Completion: zk (examination).

Teacher(s)

Guaranteed by

Prerequisites (in Czech)

B3060 Basic genetics && NOW( B6270c Cytogenetics )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Syllabus

History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. Cell cycle and DNA content in eukaryotic cells. Mitosis, meiosis and cell division. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. Chromosomal changes. Structural chromosomal changes. Origin a significance of chromosomal aberrations. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. Human cytogenetics. Cytogenetic abnormalities in hematological malignancies and solid tumors. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations.