B8090 Gene engineering

Faculty of Science
Spring 2001
Extent and Intensity
3/0/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites (in Czech)
SOUHLAS || Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Gene engineering, its history and perspective. In vitro mutagenesis as a method for changing genes, random mutagenesis, primer extension methods, cassette mutagenesis, PCR methods of site-directed mutagenesis. Protein engineering. Optimizing the expression of cloned genes, factor affecting the gene expression in a new host. Cloning in Gram-positive bacteria. Eukaryotic vectors, selectable marker for eukaryotic cells. Transfer of recombinant DNA into eukaryotic organisms. Cloning in yeast. Gene transfer to plants. Cloning in animal cells. Transferring genes into animal oocytes, eggs, embryos and specific animal tissues. Introduction of foreign genes into mice. Transgenic organisms. Gene therapy. Application of recombinant DNA to biotechnology. The impact of recombinant DNA technology.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2000, Spring 2002.
  • Enrolment Statistics (Spring 2001, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2001/B8090