Bi8021 Genetics of microorgansims

Faculty of Science
Spring 2005
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 15:00–16:50 Bpt,01013
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from biochemistry and molecular biology (Bi4020) and general microbiology (Bi4090)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Domains Bacteria, Archea, Eucarya, their characteristics from the point of wiev of genetic analysis, universal phylogenetic tree. Mutations and isolation of mutants. The use of bacterial mutants in testing of mutagenic agents. Genetic terminology. Phenotypes often used in bacterial genetics. Plasmids and conjugation. Bacteriophages and transduction. Natural compentency and transformation. Restriction and modification of DNA. Transposons and transposition. Sequencing of whole genomes and analysis of sequences. Gene expression and regulation.
Syllabus
  • Microorganisms in the living system - domains Bakteria, Archea, Eucarya. Universal phylogenetic tree of Woese. Clasical genetic analysis. Reversed genetics. Bioinformatics. Characteristics of microorganisms suitable for genetic experiments. Spontaneous and induced mutations. The use of bacterial mutants in the testing of mutagenic agents. Genetic terminology. Phenotypes mostly used in bacterial genetics. Conjugation and conjugatible plasmids. The conjugation machinery. Mobilisation of plasmids. Formation of Hfr strains. Transfer of DNA from an Hfr to another cell, transconjugants. Mapping genes on the chromosome using Hfr crosses. Fand R plasmids. Virulent and temperate bacteriophages. General transduction. Lysogenic state and lysis of bacterial cell. Specialized transduction. Genetic analysis of phages. Construction of genetic map. Genetic transformation. Natural competency. The machinery of naturally transformable cells. Restriction and modification systems. Transposition. IS elements, composite and non-composite transposons. Mechanisms of transposition. Sequencing of whole genomes and analysis of sequences. Genome composition of archeon Methanococcus janaschii. Molecular basis of life-adaptation in extreme conditions. Regulation of gene expression of procaryots. Enzyme induction. Enzyme repression. Catabolic repression. Lactose operon and the other operons of E. coli. Atenuation. Antisens RNA. Regulation of translation.
Literature
  • Snyders L.S., Champness W. ed., Molecular Genetics of Bacteria, ASM Washington, 1997
  • Rosypal S., Molekulární biologie, Díl čtvrtý, Brno, 2001
  • Trun N., Trempy J., Fundamental Bacterial Genetics, Blackwell Publishing, UK, 2004
Assessment methods (in Czech)
Výuka předmětu je ukončena zkouškou.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.
  • Enrolment Statistics (Spring 2005, recent)
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