PřF:Bi8313 Genetic engineering - practice - Course Information
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2005
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW ( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
- Syllabus
- Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
- Literature
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
- Enrolment Statistics (Spring 2005, recent)
- Permalink: https://is.muni.cz/course/sci/spring2005/Bi8313