PřF:C4220 Biochemistry-lab.course - Course Information
C4220 Biochemistry - laboratory course
Faculty of ScienceSpring 2005
- Extent and Intensity
- 0/0/7. 7 credit(s). Type of Completion: graded credit.
- Teacher(s)
- RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
prof. RNDr. Vladimír Mikeš, CSc. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor) - Guaranteed by
- RNDr. Pavla Boublíková, CSc.
Chemistry Section – Faculty of Science - Timetable of Seminar Groups
- C4220/01: Mon 7:00–12:50 Cpl,02022, P. Boublíková
C4220/02: Fri 7:00–12:50 Cpl,02022, P. Boublíková
C4220/03: Thu 7:00–12:50 Cpl,02022, T. Kašparovský
C4220/04: Wed 9:00–14:50 Cpl,02022, O. Janiczek
C4220/05: Thu 14:00–19:50 Cpl,02022, M. Mandl - Prerequisites
- C3181 Biochemistry I
Basic knowledge of Biochemistry I - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 28 fields of study the course is directly associated with, display
- Course objectives
- The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.
- Syllabus
- Qualitative reactions of carbohydrates. Determination of glucose using a biosensor. Carbohydrate thin-layer chromatography.
- Isolation and identification of lipids from the egg yolk.
- Protein electrophoresis of the blood serum. Determination and chromatographic separation of leaf pigments. Gel permeation chromatography in separation of biomolecules.
- Chemical reactions of amino acids and proteins. Determination of amino acids and peptides. Paper chromatography of amino acids. Protein isolation.
- Isolation and properties of nucleic acids.
- Heat dependence of enzyme reaction.
- Protein determination.
- pH effect on enzyme reaction. Determination of the rate of enzyme reaction.
- Substrate specifity of enzymes. Determination of enzyme activity.
- Enzyme kinetics. Enzyme inhibition.
- Enzymatic determination of metabolites.
- Electron transport chain of aerobic organisms.
- Closing test.
- Literature
- MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
- VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
- VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
- Assessment methods (in Czech)
- První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
General note: Vyučují učitelé katedry biochemie.
- Enrolment Statistics (Spring 2005, recent)
- Permalink: https://is.muni.cz/course/sci/spring2005/C4220