C8024 Separation methods B

Přírodovědecká fakulta
jaro 2011
Rozsah
2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
Vyučující
doc. Mgr. Jan Havliš, Dr. (přednášející)
Garance
doc. Mgr. Jan Havliš, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta
Předpoklady
C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited.
Omezení zápisu do předmětu
Předmět je otevřen studentům libovolného oboru.
Cíle předmětu
main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
Osnova
  • separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
  • gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
  • electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
Literatura
  • Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
  • Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
  • PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
  • AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
Výukové metody
the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
Metody hodnocení
oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
Vyučovací jazyk
Angličtina
Další komentáře
Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
Předmět je zařazen také v obdobích jaro 2011 - akreditace, jaro 2009, jaro 2010, jaro 2012, jaro 2012 - akreditace, jaro 2013, jaro 2014, jaro 2015, jaro 2016, jaro 2017, jaro 2018, jaro 2019, jaro 2020, jaro 2021, jaro 2022, jaro 2023, jaro 2024, jaro 2025.