PřF:Bi6120c Plant Tissue&Organ Culture -pr - Course Information
Bi6120c Plant Cell, Tissue and Organ Culture - practical course
Faculty of ScienceSpring 2022
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (seminar tutor)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 10:00–11:50 Kontaktujte učitele
- Prerequisites (in Czech)
- NOW( Bi6120 Plant Tissue and Organ Culture )
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cell Biology (programme PřF, B-EMB)
- Special Biology (programme PřF, B-EXB)
- Special Biology (programme PřF, B-EXB, specialization Experimental Biology of Plants)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- Passing through this course, students are able to:
apply basic methods and techniques of plant cell, tissue and organ culture in vitro;
propose in vitro methods suitable for plant multiplication, breeding and production;
estimate efficiency of the selected method for specific plant material on the base of visible symptoms. - Learning outcomes
- At the end of this course, students should be able to:
- work in sterile conditions of in vitro cultures;
- prepare culture media and sterilize them;
- create and maintain different in vitro cultures;
- transfer plants from in vitro to glasshouse. - Syllabus
- 1. Conditions of in vitro Culture, Laboratory Design.
- 2. Media Preparation and Sterilization.
- 3. In vitro Vegetative Propagation: Micropropagation by Proliferation of Axillary Buds, Adventitious Shoot Proliferation; Initiation of Primary Culture, Subculture. Transfer to ex vitro conditions.
- 4. Callus Culture: Callus Initiation and Maintenance, Subculture, Organogenesis from Callus.
- 5. Meristem Culture: Apical and Axillary Meristem Isolation and Culture.
- 6. Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos. 7. Somatic embryogenesis: induction of somatic embryos from different materials 8. Ploidy reduction: androgenesis: initiation of isolated anthers culture
- 9. Cryopreservation: Alginate Encapsulation/Dehydration of isolated axillary potato meristems.
- 10. Fungi Culture in vitro: Suspension Culture of Wood-Destroying Fungi.
- 11. Indexing and Certification of in vitro Cultures
- Literature
- SMITH, Roberta H. Plant tissue culture : techniques and experiments. Third edition. Amsterdam: Elsevier, 2013, xvi, 188. ISBN 9780124159204. info
- KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
- PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
- GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
- In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
- SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press, 2003, xxii, 346. ISBN 0199254680. info
- Phytohormones in plant biotechnology and agriculture : proceedings of the NATO-Russia Workshop held in Moscow, 12-16 May 2002. Edited by Ivana Macháčková - Georgy A. Romanov. Dodrecht: Kluwer Academic Publishers, 2003, xiv, 265. ISBN 1402017235. info
- Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons, 2006, xii, 303. ISBN 0470021810. info
- Teaching methods
- Practical course in laboratory of in vitro cultures.
- Assessment methods
- Protocols of experiments, credit.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
- Enrolment Statistics (Spring 2022, recent)
- Permalink: https://is.muni.cz/course/sci/spring2022/Bi6120c