Bi8080c Plant molecular physiology - practice

Faculty of Science
Spring 2025
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
doc. Mgr. Markéta Šámalová, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Markéta Šámalová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 17. 2. to Sat 24. 5. Wed 10:00–11:50 C13/112
Prerequisites (in Czech)
NOW( Bi8080 Plant molecular physiology )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This course builds upon Bi8080 Molecular Physiology of Plants, in which students became familiar with key mechanisms of plant function at the molecular and cellular levels. It further develops knowledge towards the application of molecular biology approaches in basic plant research and biotechnological applications. The course will focus on fundamental molecular biology methods used in the generation of genetically modified organisms (GMOs), including transgenic plants, as well as targeted genome editing in plants, using CRISPR/Cas9 technology.
Learning outcomes
After completing this course, the student will acquire theoretical and practical knowledge and be able to: • understand and describe the main methods used in molecular biology for the preparation of GMOs • master the preparation of transgenic plants and their analysis, including fluorescence and confocal microscopy • understand and analyze benefits and risks of modern plant biotechnologies.
Syllabus
• Basic procedure for the preparation of genetically modified plants • Genomic databases, primer design, PCR • Isolation of plasmid DNA, its cleavage and electrophoresis • Basics of cloning • Techniques for the transformation of E. coli and agrobacteria • Transformation of tobacco by the in vitro method • Transformation of Arabidopsis thaliana by the floral dip method • Chemically inducible systems, GUS staining • Selection of transformants • Fluorescent proteins and fluorescence microscopy • Method of targeted genome modification (CRISPR/Cas9) • Transient expression in tobacco and confocal microscopy • Benefits and risks of GMOs, legislation.
Literature
    required literature
  • ŠÁMALOVÁ, Markéta; C. KIRCHHELLE and I. MOORE. Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species. 2019. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20089. URL info
    recommended literature
  • ALBERTS, Bruce; Alexander JOHNSON; Julian LEWIS; David Owen MORGAN; Martin C. RAFF; Keith ROBERTS and Peter WALTER. Molecular biology of the cell. Edited by John H. Wilson - Tim Hunt. Sixth edition. New York, NY: Garland Science, 2014, xxxiv, 134. ISBN 9780815344322. info
  • SAMBROOK, Joseph and David W. RUSSELL. Molecular cloning : a laboratory manual. 3rd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2001, 1 sv. ISBN 0879695765. info
    not specified
  • Editing plant genomes with CRISPR/Cas9. Belhaj, K., Chaparro-Garcia, A., Kamoun, S., Patron, N.J. and Nekrasov, V. Current Opinion in Biotechnology 2015, 32:76–84.
Teaching methods
praktická výuka
Assessment methods
Test.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2026.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2025/Bi8080c