Bi1050 Biological techniques

Faculty of Science
Spring 2026
Extent and Intensity
0/2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. Mária Seifertová, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Mária Seifertová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Mária Seifertová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science
Prerequisites (in Czech)
! Bi2302 Zoological microtechnique && ! Bi1301 Microscopic Techniques && (!NOW( Bi2302 Zoological microtechnique )) && (!NOW( Bi1301 Microscopic Techniques ))
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to familiarise students with basic biological techniques and with the methods of processing biological material. Special emphasis is placed on acquiring practical skills essential for conducting laboratory and demonstration-based biology teaching at primary and secondary schools – from working with a light microscope to preparing simple instructional and experimental specimens.
Learning outcomes
At the end of this practical course, students should be able - to use various techniques of light microscopy efficiently; - to prepare various kinds of zoological mounts; - to conduct simple biological experiment.
Syllabus
Native preparations. Vital staining. Whole mounts, sections, squashes, dry mounts. Fixation. Fixatives. Staining. Aqueous media. Non-aqueous mounting media.  Histological technique. Documentation. Fluorescence. Preparation.
Literature
    recommended literature
  • KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
  • LELLÁKOVÁ, Františka. Zoologická technika. 1. vyd. Praha: Universita Karlova, 1985, 122 s. info
  • Murphy, D. B., & Davidson, M. W. (2012). Fundamentals of light microscopy and electronic imaging. John Wiley & Sons.
  • Matis, D. a kol. (2001): Mikroskopická technika. Faunima, Bratislava.
  • Hejtmánek, M. (2001): Úvod do světelné mikroskopie. Skripta UP Olomouc.
    not specified
  • Wayne, R. O. (2019). Light and video microscopy. Academic Press.
Teaching methods
Practical training.
Assessment methods (in Czech)
Kurz je ukončen zápočtem.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024, Autumn 2025.
  • Enrolment Statistics (recent)
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