aFAFO1_12 Phytochemistry

Farmaceutická fakulta
podzim 2025

Předmět se v období podzim 2025 nevypisuje.

Rozsah
1/1/0. 14p+14c. 3 kr. Ukončení: z.
Vyučující
prof. PharmDr. Karel Šmejkal, Ph.D. (přednášející)
Ing. Ondřej Jurček, Ph.D. et Ph.D. (cvičící)
prof. PharmDr. Karel Šmejkal, Ph.D. (cvičící)
Garance
prof. PharmDr. Karel Šmejkal, Ph.D.
Ústav přírodních léčiv – Ústavy – Farmaceutická fakulta
Předpoklady
The students should have passed basic subjects touching organic chemistry, analytical chemistry, biology, botany, and physiology.
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
The course examines the relationships between primary and secondary metabolism of plants and focuses on the study of secondary metabolites, their biosynthesis, properties, chemical structure, and methods of isolation from plant material. In this course, students will get acquainted with the methods of phytochemical analysis
Výstupy z učení
Basic knowledge of secondary metabolites and methods for their analysis.
Osnova
1 Phytochemistry as the field of science, primary and secondary metabolism.
2 Principles of separation and isolation of natural substances.
3 Systems for classification of natural compounds, secondary metabolites.
4 Chromatography I.
5 Chromatography II.
6 Identification of Natural Products
7 High-performance liquid chromatography, gas chromatography
8 Spectral methods for identifying natural compounds (UV, IR).
9 Spectral methods for identifying natural compounds (MS).
Laboratory of phytochemistry
1 Introduction, safety, theoretical basics of methods for obtaining content substances from plants
2 Isolation of Natural Products - Floral pigment of Urticae herba extraction (Soxhlet), caffeine microsublimation, Species urologicae compounds extraction (maceration), hydrodistillation of essential oils
3 Separation of natural substances - Thin layer chromatography of floral pigments of Urticae herba extract and phenolic glycosides from the extract of Species urologicae
4 Separation of Natural Products II - Column chromatography of floral pigments
5 Separation and identification of natural substances
Determination of carotenoid pigments by spectrophotometry, measuring of the melting point of crystalline natural substances, SFE demonstration, IR spectrometry demonstration.
6 Identification of Natural Products
High-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), HPLC / MS,
7 Final test
Literatura
    povinná literatura
  • Smejkal, K, Muselik, J, Mokry, P. Laboratory methods of experimental phytochemistry. Brno, 2017. ISBN 978-80-7305-796-1. info
    doporučená literatura
  • From herbs to healing : pharmacognosy - phytochemistry - phytotherapy - biotechnology. Edited by Éva Szöke - Ágnes Kéry - Éva Lemberkovics. Cham: Springer, 2023, xvii, 570. ISBN 9783031173004. info
  • Assessment of medicinal plants for human health : phytochemistry, disease management, and novel applications. Edited by Megh Raj Goyal - Durgesh Nandini Chauhan. Burlington, ON: Apple Academic Press, 2021, xxiii, 261. ISBN 9781771888578. info
Výukové metody
lectures, laboratory exercises
Metody hodnocení
written test
Vyučovací jazyk
Angličtina
  • Permalink: https://is.muni.cz/predmet/pharm/podzim2025/aFAFO1_12