FAFY1_12 Phytochemistry

Farmaceutická fakulta
jaro 2025
Rozsah
1/1/0. 3 kr. Ukončení: z.
Vyučováno kontaktně
Vyučující
prof. PharmDr. Karel Šmejkal, Ph.D. (přednášející)
Ing. Ondřej Jurček, Ph.D. et Ph.D. (cvičící)
Mgr. Patrik Sivulič (cvičící)
Garance
prof. PharmDr. Karel Šmejkal, Ph.D.
Ústav přírodních léčiv – Ústavy – Farmaceutická fakulta
Předpoklady
The students should have passed basic subjects touching organic chemistry, analytical chemistry, biology, botany, and physiology.
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.

Předmět si smí zapsat nejvýše 24 stud.
Momentální stav registrace a zápisu: zapsáno: 0/24, pouze zareg.: 0/24
Mateřské obory/plány
Cíle předmětu
The subject investigates the relationships of the primary and secondary metabolism of the plants and is directed on the study of the secondary metabolites, their biosynthesis, their properties, chemical structure, and the ways of their isolation from the plant material. In the scope of this subject, students get acquainted with important methods of phytochemical analysis.
Výstupy z učení
Basic knowledge of secondary metabolites and methods for their analysis.
Osnova
  • 1 Phytochemistry as the field of science, primary and secondary metabolism.
    2 Principles of separation and isolation of natural substances.
    3 Systems for classification of natural compounds, secondary metabolites.
    4 Chromatography I.
    5 Chromatography II.
    6 Identification of Natural Products
    7 High-performance liquid chromatography, gas chromatography
    8 Spectral methods for identifying natural compounds (UV, IR).
    9 Spectral methods for identifying natural compounds (MS).
    Laboratory of phytochemistry
    1 Introduction, safety, theoretical basics of methods for obtaining content substances from plants
    2 Isolation of Natural Products - Floral pigment of Urticae herba extraction (Soxhlet), caffeine microsublimation, Species urologicae compounds extraction (maceration), hydrodistillation of essential oils
    3 Separation of natural substances - Thin-layer chromatography of floral pigments of Urticae herba extract and phenolic glycosides from the extract of Species urologicae
    4 Separation of Natural Products II - Column chromatography of floral pigments
    5 Separation and identification of natural substances
    Determination of carotenoid pigments by spectrophotometry, measuring of the melting point of crystalline natural substances, SFE demonstration, IR spectrometry demonstration.
    6 Identification of Natural Products
    High performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), HPLC / MS,
    7 Final test
Literatura
    doporučená literatura
  • Evans W Ch. Trease and Evans' Pharmacognosy. 2009. ISBN 9780702029332. info
  • Dewick P. Medicinal natural products, a biosynthetic approach. Hoboken, 2008. ISBN 9780470741689. info
  • Walton NJ, Brown DE. Chemicals form plants. 1999. ISBN 978-981-4498-52-4. info
  • Bruneton Jean. Pharmacognosy, Phytochemistry, Medicinal Plants. 1999. ISBN 1898298637. info
Výukové metody
lectures, practical exercises
Metody hodnocení
Written test
Vyučovací jazyk
Angličtina
Informace učitele
100% attendance of all the practices
Passing the semester tests and the final exam (60% of the total is required)
Další komentáře
Předmět je dovoleno ukončit i mimo zkouškové období.
Předmět je zařazen také v obdobích jaro 2020, jaro 2021, jaro 2022, jaro 2023, jaro 2024.