FABC1_16 Biochemistry

Farmaceutická fakulta
podzim 2021
Rozsah
3/1/0. 6 kr. Ukončení: zk.
Vyučující
Mgr. Marie Brázdová, Ph.D. (přednášející)
Mgr. Marek Petr, Ph.D. (přednášející)
PharmDr. Zuzana Soldánová (přednášející)
Mgr. Marie Brázdová, Ph.D. (cvičící)
Mgr. Marek Petr, Ph.D. (cvičící)
PharmDr. Zuzana Soldánová (cvičící)
Mgr. Magdaléna Drotárová (cvičící)
Ing. Branislav Ruttkay-Nedecký, Dr. (cvičící)
Garance
Mgr. Marie Brázdová, Ph.D.
Ústav molekulární farmacie – Ústavy – Farmaceutická fakulta
Rozvrh
každý sudý pátek 12:00–17:00 45-224
  • Rozvrh seminárních/paralelních skupin:
FABC1_16/01: Pá 24. 9. až Pá 22. 10. každý sudý pátek 8:00–11:20 45-323; a Pá 5. 11. 8:00–9:40 45-323
Předpoklady
FAKULTA(FaF) || OBOR(MUSFaF)
The knowledge of subject in range of lectures
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
Biochemistry is the study of the chemical processes (changes) which condition and accompany life in its most varied forms and manifestations, and attemps to explain its essence on a molecular level. Medical biochemistry is biochemistry in use, applied quite specifically in veterinary medicine (for example, the peculiarities of the metabolism of nutrients in invidual species of livestock). Further, in teaching of biochemistry, it is necessary to concentrate on detailed interpretation of selected chapters, that are most significant for the formation of the European profile of a graduate of veterinary university. Practical (laboratory) work where verbal information is confirmed by practical experience is also an integral component of the biochemistry teaching.
Výstupy z učení
Basic knowledge of biochemistry forming the basis for follow-up subjects Methods of molecular biology, Biotechnology of drugs, Exercises from drug biotechnology, Pharmacogenomics, Structure and evolution of genomes.
Osnova
  • Syllabus - Biochemistry 1) Biochemistry: an introduction 1.1) Scope of biochemistry: central principles, cellular and chemical foundations (functional groups, reactions), physical foundations 1.2) Living cells (structure of eukaryotic cell) 1.3) Water: the medium of life (interactions, pH, pKa, buffers, blood, liver and urine buffering systems, osmosis, dialysis) 2) Structure and function of proteins 2.1) Amino acids (structure, pKa, pI, properties, covalent modifications) 2.2) Peptides (peptide bound, disulfide bridge, glutathione, insulin, antibiotics, neurotransmiters) 2.3) Protein structure (secondary, tertiary, conformation, structure and function) 2.4) Structure and function of hemoglobin and myoglobin 3) Enzymes 3.1) Properties of Enzymes (cofactors, coenzymes, vitamins) 3.2) Classification of Enzymes (examples of each class) 3.3) Catalysis (enzyme reaction, active site, specificity) and Enzyme regulation 3.4) Kinetics of enzyme reactions 4) Membrane transport 4.1) Biomembranes - composition and function 4.2) Membrane transports 5) Carbohydrates and metabolism of carbohydrate 5.1) Monosaccharides, Disaccharides, Oligosaccharides 5.2) Polysaccharides (structure and function) 5.3) Metabolism, homeostasis, catabolism, anabolism, nutrient pool, energy metabolism 5.4) Glycolysis and Glyconeogenesis 5.5) Glycogen metabolism 6) Metabolism of Proteins and Amino Acids 6.1) Overview of metabolism of proteins 6.2) Elimination of alfa-amino nitrogen 6.3) Detoxification of Ammonia 6.4) Catabolism and Anabolism of Amino Acids (Ala, Arg, Ser, Gly, Thr, Asp, Glu, His, Phe, Tyr, Cys) 7) Aerobic metabolism 6.1) Tricarboxy Acid Cycle (Citric Acid Cycle (overview, fuels, mitochondrion, enzymes and coenzymes, 8 steps of CC, regulation of pyruvate dehydrogenase, control of CC, biosynthesis aspects of CC) 6.2) Respiratory chain (redox potential, sequence of electron carriers) 6.3) Oxidative phosphorylation (enzymes, prosthetic groups, localization in mitochondria, mechanism ATP synthesis) 6.4) Oxidative stress (reactive oxygen species, their formation, antioxidant enzymes systems, antioxidant molecules) 7) Lipids 7.1) Lipids classes (definition, biological) and metabolism 7.2) Synthesis and degradation of Fatty acids 7.3) Lipid synthesis. Peroxidation and Eicosanoids. 7.4 Biotransformation 8) Nucleic acids and their metabolism 8.1) Structure of components, role of nucleotides, biosynthesis (replication, transcription and proteosynthesis 8.2) Nucleic acids metabolism (biosynthesis and degradation of purine and pyrimidine nucleotides) 9) Integration of metabolism and cell signaling Exercise No. 1: Determination of glucose concentration using spectrophotometric method and diagnostic strips. Exercise No. 2: Spectrophotometric determination of magnesium concentration using the calibration line method. Exercise No. 3: Chromatographic, electrophoretic and spectral methods for the stage of properties of nucleic acids and proteins. Exercise No. 4. Determination of catalytic concentration of lactate dehydrogenase. Kinetics of enzyme reactions.
Literatura
    povinná literatura
  • J. Tomandl. Biochemistry I. Brno, 2012. ISBN 978-80-210-5741-8. info
  • R.K.Murray, D.A.Bender, P.J.Kennelly, V.W. Rodwell, P.A.Weil. Harper´s illustrated biochemistry. USA, 2012. ISBN 978-0-07-179277-6. info
    doporučená literatura
  • J. Tomandl. Biochemistry I. Brno, 2012. ISBN 978-80-210-5741-8. info
  • J.W. Pelly. Elsevierś integrated review Biochemistry. Philadelphia, 2012. ISBN 978-0-323-07446-9. info
  • MURRAY,R.K., GRANNER,D.K.,RODWELL,V.W. Harper´s Illustrated Biochemistry. Appleton & Lange, 2006. ISBN 07-147885-X. info
  • A. L. Lehninger, D. L. Nelson, M. M. Cox. Principles of biochemistry. USA, 2005. ISBN 0716743396. info
  • T.McKee, J.S.McKEe. Biochemistry. USA, 1996. ISBN 0-697-21159-2. info
Výukové metody
lectures, class discussion, laboratory work, homework,
Metody hodnocení
Requirements: Lectures: - Weekly tests - An exercise book: Seminars from Biochemistry " - Assessment of the practical course The exam will have two parts: - Exam test - minimum 60% - Oral examination, both parts must be carried out in one semester Practical exercises: - 100% attendance - Weekly test of the executed task - The final credit test - minimum 80% The written part, which consists of a test with 40 questions, 150 points. To successfully complete the course, it is necessary to pass the test at 60%. Prerequisite for passing the exam is obtaining credit, to obtain credit from the subject "BIOCHEMISTRY" it is necessary to complete all practical exercises, passing a credit test at 80%, adequate knowledge of biochemical issues and the correct elaboration of all protocols for exercises. At the same time, a short task readiness test will be performed before each exercise. During the study it is mandatory to pass 4 tests from basic biochemical pathways. Furthermore, the group task of elaborating 2 chapters of seminars. The written exam is prepared on the basis of lectures and questions from the Seminars - see recommended literature. He will then reimburse the teaching at the time and in the manner determined by the respective teacher. If the credit is not awarded, the student will perform the credit procedure in the term determined by the relevant teacher of practical training. If the prescribed completion of the course is only a credit, the number of terms for obtaining it is identical, as if the course was completed by an exam.
Vyučovací jazyk
Angličtina
Informace učitele
Grading Procedure:
Lectures:- Weekly quiz covering the topic from the lectures
- Preparation of Seminars from Biochemistry
- Credit from practical course
Exam - two parts:
- Exam test minimum of 60%
- Oral examination: both exam test and oral examination in one semester
Practical course:
- 100% of presence on practical courses
- Weekly quiz covering the topic of the practical course
- Final credit test from practical course 80%
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