Bi0210 Plant Gene Engineering

Faculty of Science
Autumn 2011
Extent and Intensity
2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Ladislav Havel, CSc. (lecturer)
Guaranteed by
prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Ladislav Havel, CSc.
Prerequisites (in Czech)
Bi8240 Plant genetics && Bi8090 Gene engineering
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
Seznámit studenty s principy a aplikacemi metod genového inženýrství u rostlin. Tj. s manipulacemi s nukleovými kyselinami a rostlinami za účelem využívání těchto postupů při měnění genotypu a fenotypu rostlin a rozšiřování spektra možností využívání rostlin v teoretickém výzkumu a především zemědělské, potravinářské, farmaceutické a medicínské praxi).
Syllabus
  • 1. Plant genome structure. Plant genome complexity, nuclear, mitochodndrial and plastid DNA. Plant chromosome structure. 2. Principles of DNAs manipulations. Isolation and separation of nucleic acids Hybridization of nucleic acids. Nucleic acids sequence detection. 3. Polymerase chain reaction. Principles. Special procedures - RT-PCR, RAPD, RACE-PCR, AFL. 4. Plant transgenosis. Vector types (plasmids, fags, artificial chromosomes). Agrobacterium tumefaciens, A. rhizogenes - Ti-plazsmid, RI-plasmid a T-DNA. T-DNA transfer to plant cell. Detection of T-DNA transfer. 5. Transgenic (genetically modified) plants. Herbicide resistance. Insects resistance. Virus resistance. Increased metabolic compounds production. 6. Advantages and risks joined with the use of genetically modified plants in agriculture. Decrease of environmental stress. New therapy possibilities. Decrease of genetic variability. 7. Legislation connected to genetically modified plants Distribution of growing genetically modified plants in the world and their production. Legislation in the EU. Legislation in Czechia and Slovakia. Legislation in the other parts of world.
Literature
    required literature
  • RELICHOVÁ, Jiřina. Genové inženýrství rostlin (Genetic engineering of plants). Slovo. Praha: Melantrich, 1997, vol. 1997, 22.3.1997, p. 5. ISSN 0231-732X. info
    recommended literature
  • VONDREJS, Vladimír. Otazníky kolem genového inženýrství. Vyd. 1. Praha: Academia, 2010, 134 s. ISBN 9788020018922. info
  • LODISH, Harvey F. Molecular cell biology. 5th ed. New York: W.H. Freeman and Company, 2004, xxxiii, 97. ISBN 0-7167-4366-3. info
  • ROSYPAL, Stanislav, Jiří DOŠKAŘ, Karel PETRZIK and Vladislava RŮŽIČKOVÁ. Úvod do molekulární biologie IV. Molekulární biologie rostlinných virů, Priony, Molekulkární evoluce, Vznik života, Metody molekulární biologie, Genové inženýrství (Introduction to molecular biology IV.). Třetí inovované vydání. Brno: Rosypal S., Grafex, 2002, 300 pp. Díl čtvrtý. ISBN 80-902562-4-4. info
Teaching methods
lectures, discussions, DVD presentations
Assessment methods
oral examination
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011 - acreditation.
  • Enrolment Statistics (recent)
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