PřF:Bi5850 Modern lab methods in medicine - Course Information

Bi5850 Application of modern laboratory methods in medicine

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Šárka Pospíšilová, Ph.D. (lecturer)
Ing. Ivana Ježíšková, Ph.D. (lecturer)
doc. Mgr. Martina Lengerová, Ph.D. (lecturer)
Ing. Martina Almáši, Ph.D. (lecturer)
prof. MUDr. Michael Doubek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Šárka Pospíšilová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

( Bi1700 Cell Biology ) && ( Bi4010 Essential molecular biology || Bi4020 Molecular biology )
Bi1700 Bi4010 Bi4020

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10

fields of study / plans the course is directly associated with

there are 11 fields of study the course is directly associated with, display

Course objectives

Teaching takes place in Center for Molecular Biology and Gene Therapy IHOK LF MU / FN Brno and Department of Clinical Hematology (OKH) and combines lectures by current molecular biological approaches used in medical practice, especially in cancer diagnostics, with practical examples of analysis of patient samples. Student is able to describe the application of molecular biological methods in hemato-oncology and to apply this knowledge in ongoing studies and in practice.

Learning outcomes

Student is able to use selected molecular biological methods in hemato-oncology and to apply this knowledge in ongoing studies and in practice.

Syllabus

• Theoretical Background: The use of molecular genetics in oncology and hematology modern methods of analysis of the human genome • Practical part: isolation of DNA / RNA from blood, immunomagnetic separation of blood cells, PCR techniques and real time PCR, detection of minimal residual disease, detection of infectious pathogens using PCR, DNA next-generation sequencing – analysis of exomes and amplicones, immunofluorescent labeling using monoclonal antibodies, flow cytometric analysis of T-and B-lymphocytes from peripheral blood, other techniques according to the interest and ability of laboratories.

Literature

recommended literature
• Culture of human tumor cells. Edited by Roswitha Pfragner - R. Ian Freshney. Hoboken, New Jersey: Wiley-Liss, 2004, xvi, 435. ISBN 0471438537. info
• SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info

Teaching methods

Lectures documenting significance of the molecular-biology approaches in medicine, especially in oncology, are combined with practical demonstrations of specific methods.

Assessment methods

Credits are assigned for active participation in the course and rigorous writing of protocols.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught each semester.
The course is taught: in blocks.
Note related to how often the course is taught: dva identické bloky v termínech 29.10.-2.11. 2018 a 14.1.-18.1.2019.
General note: Primárně určeno pro studenty zpracovávající závěrečné práce v oboru molekulární hematologie, onkologie a lékařské genomiky.
Information on course enrolment limitations: (Bi2080 || Bi5800 || Bi1700) && (Bi4010 || Bi4020c) && Bi6400 && Bi6405 && (Bi5000 || NOW(Bi5000))

• Enrolment Statistics (recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2018/Bi5850