Bi6721 Special Methods of Microorganisms Analysis

Faculty of Science
Spring 2023
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Taught in person.
Mgr. Pavel Dvořák, Ph.D. (lecturer)
Guaranteed by
Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Wed 13:00–14:50 E25/209
The course is destined for students of microbiology and other students who are interested in new methods of analysis of microorganisms. The prerequisite is the passed exam of the course Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the principles of selected methods of molecular biology, biochemistry, and biophysics for analysis of microorganisms, with the procedure for selection of a suitable method for solving a real problem, differences in procedures used for bacteria and yeasts and interpreting the results of analyzes. Students will receive specific information about the following processes: 1)Isolation of nucleic acids (NA) from microorganisms and analysis of NA using chromatographic and electrophoresis methods 2)Application of enzymes in microbial genes analysis 3)Sequence analysis of microbial genes, genomes, and proteins 4)Application of hybridisation and polymerase chain reaction in microbiology 5)Methods of study of microbial proteins and metabolites 6)Application of recombinant NA for the study of microorganisms
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology, biochemistry and biophysics methods used in microbiology, propose the procedure for solving a real problem, realize differences between methods used for bacteria and yeasts; interpret the results.
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
  • Methods for preparation of recombinant DNA and RNA molecules and their use for the study of microorganisms.
    recommended literature
  • Persing D.H. a kol. Molecular Microbiology: Diagnostic Principles and Practice, 3rd Edition. 2016, 835 p. ISBN: 978-1-555-81908-8
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • Brown T.A. Gene Cloning and DNA Analysis: An Introduction, 8th Edition. 2020, Wiley-Blackwell, 432 p. ISBN: 978-1-119-64078-3
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005. 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of Powerpoint presentations. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answer 15 questions. The overall result of the exam is evaluated by the examiner. Test if followed by a brief discussion with the examiner (oral part of the exam).
Language of instruction
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022.
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