Bi7430c Molecular biotechnology - practical course

Faculty of Science
Autumn 2024
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
Mgr. David Kovář, Ph.D. (seminar tutor)
Ing. RNDr. Martin Marek, Ph.D., MBA (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Mgr. Michal Vašina, Ph.D. (seminar tutor)
Mgr. Bc. Alan Strunga (seminar tutor)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
PharmDr. Bc. Josef Mašek, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi7430c/01: Wed 9:00–10:50 C13/332, D. Kovář, Z. Prokop, M. Vašina
Bi7430c/02: Wed 14:00–15:50 C13/332, D. Kovář, Z. Prokop, M. Vašina
Prerequisites
NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the exercise, students will know a number of practical procedures of molecular biotechnology, e.g. primer design, DNA cleavage with restriction enzymes, transformation of bacterial cells, methods of fermentation cultivation of E. coli, use of recombinant technologies in biosensing and removal of dangerous substances, development of recombinant therapeutics and vaccines. The course will allow students to deepen their theoretical knowledge acquired in the Molecular Biotechnology lectures.
Learning outcomes
The exercise provides a combination of practical skills taught by PřF MUNI and VÚVeL experts. Students will get to know the currently used methods as well as the principle and operation of modern laboratory equipment. The subject also aims to strengthen students' communication skills through discussions with experts in the given field, it will also help the development of students' stylistic skills during the independent preparation of essays and protocols from completed exercises. The use of study materials in English will support the ability to work with professional texts, including consolidating knowledge of English terminology used in this field.
Syllabus
  • Advanced Molecular Biology Tools
  • Protein Expression Technologies
  • Microfluidics, Lab on a Chip
  • Drug delivery systems and recombinant vaccines
  • Development of recombinant protein therapeutics
  • Enzymatic assay for determination of environmental pollutants
  • Basics of biochemical data analysis
Literature
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Teaching methods
In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample or dataset.
The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice.
Students will receive ELECTRONIC MATERIALS for each topic (e.g., methodologies, methodological articles, video tutorials)
Assessment methods
Students will obtain positive assessment of the practice for active participation in exercises and preparation of protocols or essays from each exercise.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.
Teacher's information
https://loschmidt.chemi.muni.cz/peg/courses/
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2024/Bi7430c