Knowledge of Analytical Chemistry on a level of introductory course Knowledge of high school Physics. The course is given in Czech or English - as requested by students. The final oral examination will be in Czech or English.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
Fields of study the course is directly associated with
Student will understand fundamentals of mass spektrometry including ionization methods, mass analyzers, ion detectors and selected applications, e.g. ionization mtechniques MALDI and ESI, modern mass snalyzers time-of-flight, ion traps and orbitrap, identification and quantification of proteins.
1. A Brief Historical Perspective:
Overview of Techniques and Technology. Basic Concepts of MS (resolution, sensitivity).
2. Ionization Techniques and Sample Introduction:
Electron Impact (EI).
Chemical Ionization (CI).
Inductively Coupled Plasma (ICP).
Fast Atom Bombardment (FAB).
Secondary Ion Mass Spectrometry (SIMS).
Laser Desorption (LD).
Plasma Desorption (PD).
Matrix-Assisted Laser Desorption/Ionization (MALDI).
Coupling of Separation and MS (on-line, off-line, chips)
3. Mass Spectrometers:
Simulation of Ion Movement (Simion).
Quadrupole Mass Analyzer.
Ion Cyclotron Resonance Fourier Transform Mass Spectrometer (FT-ICR-MS).
Ion Trap (IT).
Linear Trap (LT).
Time-of-Flight Mass Spectrometer (TOFMS).
Collision-Induced Dissociation (CID).
Tandem Mass Spectrometry (MS/MS).
Vacuum Principles & Techniques.
Detectors & Detection Electronics.
4. MS Applications:
Proteins and peptides. Peptide mapping, protein databases.
COTTER, Robert J. Time-of-Flight Mass Spectrometry: Instrumentation and applications in biological research. Washington, D.C.: American Chemical Society, 1997. 326 pp. ISBN 0-8412-3474-4. info
COLE, Richard B. Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation & Applications. : John Wiley & Sons, Inc., 1997. 577 pp. ISBN 0-471-14564-5. info
Lectures and discussion in the end of the semester. Visit of a mass spectrometry laboratory, demonstrations of MS hardware.
Final oral examination (in Czech or English). The exam consists of three questions on the following topics: 1. ionization techniques, 2. mass analyzers, 3. applications. Fifty percent of correct answers is needed to pass.
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: V podzimním semestru 2011 bude předmět vyučován v anglickém jazyce. Zkoušku je možné složit v češtině.