C8202 Proteomics - a basic course

Faculty of Science
Spring 2019
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
Mgr. Gabriela Lochmanová, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research - Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: National Centre for Biomolecular Research - Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Mon 15:00–16:50 A11/335
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this subject is an introduction of proteomics as discipline dealing with characterization of proteome and individual proteins; methods for their characterization including higher levels of protein structure. To provide students with outline of methods for preparation of protein samples, basic methods for protein separation and characterization of proteins by mass spektrometry including principles of data processing.
Learning outcomes
At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (C8302) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
Syllabus
  • Introduction to proteomics Protein functions/Recombinant proteins Characterization of protein structure Basics of protein sample preparation Methods for separation of proteins and peptides Mass spektrometry (MS) in proteomics Introduction to processing of MS data
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Teaching methods
The main teaching method are lectures that include particular examples from the own scientific praxis of participating teachers and demonstration of the solution of particular problems via individual tools of recent proteomics.
Assessment methods
Lecture will be given in 2-hour units
study materials will be available in IS
written exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018.
  • Enrolment Statistics (recent)
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