FB820 Structural electron microscopy

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Taught online.
Teacher(s)
Ing. Tibor Füzik, Ph.D. (lecturer)
Mgr. Jiří Nováček, Ph.D. (lecturer)
Ing. Ondřej Sháněl, Ph.D. (lecturer), Ing. Tibor Füzik, Ph.D. (deputy)
Guaranteed by
Mgr. Jiří Nováček, Ph.D.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Prerequisites
Knowledge of English, mathematics, and physics at the grammar school level.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The course will provide an introduction to electron microscopy for structural, cell biology. Students will gain practical knowledge oriented towards understanding sample preparation, principles of electron microscope function, measurement methods and data analysis.
Learning outcomes
Upon completion of the course, students will gain:
- an understanding of the principles of the electron microscope
- understanding of the principles of image formation in electron microscopy
- ability to evaluate the suitability of electron microscopy for problem solving in biological research
- an understanding of the methods of data analysis
Syllabus
  • 1. Interaction of electron with a matter
  • 2. Anatomy of electron microscope
  • 3. Principles of image formation
  • 4. Sample preparation for life science electron microscopy
  • 5. Fourier transform
  • 6. 3DEM methods
  • 7. 3DEM methods – single particle analysis
  • 8. 3DEM methods – electron tomography
  • 9. Data interpretation
Literature
    recommended literature
  • Annie Cavalier, Daniele Spehner, Bruno M. Humbel: Handbook of Cryo-Preparation Methods for Electron Microscopy (Methods in Visualization), CRC Press; 1st Ed., 2008
  • R. A Crowther: The Resolution Revolution: Recent Advances In cryoEM, Volume 579 (Methods in Enzymology) 1st Edition, Academic Press, 2016
  • John Kuo: Electron Microscopy: Methods and Protocols (Methods in Molecular Biology), Humana Press; 3rd ed. 2014
Teaching methods
Lectures, class discussions
Assessment methods
Semester work, written examination
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (Autumn 2024, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2024/FB820