Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2015
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
doc. RNDr. Milan Bartoš, Ph.D. (lecturer), prof. RNDr. Zdeněk Hubálek, DrSc. (deputy)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Zdeněk Hubálek, DrSc.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Wed 15:00–16:50 E25/209
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, to realize differences of methods used for bacteria and yeasts; interpret the results.
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
    recommended literature
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005. 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021.
  • Enrolment Statistics (Spring 2015, recent)
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