Bi6120c Plant Cell, Tissue and Organ Culture - practical course

Faculty of Science
Spring 2023
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Hana Cempírková, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi6120c/01: Thu 10:00–11:50 C13/222, H. Cempírková
Bi6120c/02: Thu 8:00–9:50 C13/222, H. Cempírková
Prerequisites (in Czech)
NOW( Bi6120 Plant Tissue and Organ Culture )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Passing through this course, students are able to:
apply basic methods and techniques of plant cell, tissue and organ culture in vitro;
propose in vitro methods suitable for plant multiplication, breeding and production;
estimate efficiency of the selected method for specific plant material on the base of visible symptoms.
Learning outcomes
At the end of this course, students should be able to:
- work in sterile conditions of in vitro cultures;
- prepare culture media and sterilize them;
- create and maintain different in vitro cultures;
- transfer plants from in vitro to glasshouse.
Syllabus
  • 1. Conditions of in vitro Culture, Laboratory Design.
  • 2. Media Preparation and Sterilization.
  • 3. In vitro Vegetative Propagation: Micropropagation by Proliferation of Axillary Buds, Adventitious Shoot Proliferation; Initiation of Primary Culture, Subculture. Transfer to ex vitro conditions.
  • 4. Callus Culture: Callus Initiation and Maintenance, Subculture, Organogenesis from Callus.
  • 5. Meristem Culture: Apical and Axillary Meristem Isolation and Culture.
  • 6. Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos. 7. Somatic embryogenesis: induction of somatic embryos from different materials 8. Ploidy reduction: androgenesis: initiation of isolated anthers culture
  • 9. Cryopreservation: Alginate Encapsulation/Dehydration of isolated axillary potato meristems.
  • 10. Fungi Culture in vitro: Suspension Culture of Wood-Destroying Fungi.
  • 11. Indexing and Certification of in vitro Cultures
Literature
  • SMITH, Roberta H. Plant tissue culture : techniques and experiments. Third edition. Amsterdam: Elsevier, 2013, xvi, 188. ISBN 9780124159204. info
  • KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
  • PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
  • GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
  • In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
  • SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press, 2003, xxii, 346. ISBN 0199254680. info
  • Phytohormones in plant biotechnology and agriculture : proceedings of the NATO-Russia Workshop held in Moscow, 12-16 May 2002. Edited by Ivana Macháčková - Georgy A. Romanov. Dodrecht: Kluwer Academic Publishers, 2003, xiv, 265. ISBN 1402017235. info
  • Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons, 2006, xii, 303. ISBN 0470021810. info
Teaching methods
Practical course in laboratory of in vitro cultures.
Assessment methods
Protocols of experiments, credit.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, Spring 2019, Spring 2021, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/Bi6120c