Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Tue 8:00–9:50 Bpt,01013
Prerequisites
The course is for the students with interest in advanced molecular genetic and diagnostic methods used in analysis of microorganisms. Prerequisits are exams from Melecular Biology (Bi4020) and General mikrobiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Methods of the study of microbial cells - cultivation and noncultivation methods. DNA probes (characterisation and use,.labelling and detection). Experimental arrangements during hybridisations (hybridisations in solution and on solid phases, fluorescent in situ hybridisation). Microchips, their construction and use in microbiology. Amplification methods in molecular diagnostics of microorganisms (amplification of target DNA sequence, probe, signal carried on a probe). Real time PCR and detection of PCR products, (SybrGreen, TaqMan probes, hybridisation probes, molecular beacons). Enzymes used for manipulation with nucleic acids and thier use in construction of restriction maps. Molecular typisation methods in mikrobiology based on DNA and protein analysis. Methods of sequencing of nucleic acids and analysis of DNA sequences. Metagenomics - analysis of microbial communities. Molecular diagniostics of microorganisms with use of magnetic carriers. Search for biologically active compounds using of microorganisms. Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
At the end of this course the student will be able to understand the use of advanced molekular genetic methods in mikrobiology, to find suitable method for solving of conrete problem and to interpret results of analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
Assessment methods
Lectures are enclosed by oral exam.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/Bi7722