C8857 Study of interactions between proteins and DNA

Faculty of Science
Autumn 2008
Extent and Intensity
1/0/0. 4 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Lumír Krejčí, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Lumír Krejčí, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Timetable
Thu 10:00–10:50 C04/211
Prerequisites
The course will be ended by test. Each student will have to also deliver home assignment that describes their particular project with the potential implementation of the methods and techniques discussed during the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 15 fields of study the course is directly associated with, display
Course objectives
1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II
Syllabus
  • 1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II
Literature
  • MEYERS., Robert A. Encyclopedia of Molecular Cell Biology and Molecular Medicine, 2nd Edition. 2004. ISBN 3-527-30545-9. info
  • BRANDEN, Carl and John TOOZE. Introduction to Protein Structure. 1991. ISBN 0-8153-0270-3. info
Assessment methods
The course will be ended by test. Each student will have to also deliver home assignment that describes their particular project with the potential implementation of the methods and techniques discussed during the course.
Language of instruction
English
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/C8857