IB024 Mapping the Arabidopsis core cell cycle machinery by targeted interactomics

Přírodovědecká fakulta
podzim 2010 - akreditace
Rozsah
0/0. 2 kr. Ukončení: z.
Vyučující
prof. Geert de Jaeger (přednášející)
Garance
doc. RNDr. Jan Hejátko, Ph.D.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta
Kontaktní osoba: doc. RNDr. Jan Hejátko, Ph.D.
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Osnova
  • The mapping of protein interactions is a popular method for gene discovery, functional gene analysis or the study of gene networks. Yeast two-hybrid or protein complementation assays are used to map binary interactions, but the emergence of powerful, ultrasensitive high-throughput mass spectrometry (MS), together with the availability of comprehensive protein sequence repertoires, has favored the development of methods relying on in situ affinity purification of protein complexes. Especially, the tandem affinity purification (TAP) approach, based on the expression of a bait protein fused to a double affinity tag, has proven to be of great value. We developed a high throughput TAP technology platform for complex isolation from Arabidopsis cell suspension cultures (1,2) and applied it to map the core complex machinery at the heart of the Arabidopsis cell cycle control. The resulting network offers for the first time a comprehensive view on CDK/cyclin complexes of a higher plant and contains over 100 new candidate cell cycle proteins. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Furthermore, we could demonstrate that plants have evolved a combinatorial toolkit comprising at least a hundred different CDK/cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants.
Vyučovací jazyk
Angličtina
Informace učitele
Geert De Jaeger Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), Ghent University, Technologiepark 927, B-9052 Gent
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