Bi7533c Soil ecotoxicology - laboratory course

Faculty of Science
Autumn 2010
Extent and Intensity
0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
prof. RNDr. Jakub Hofman, Ph.D. (seminar tutor)
Mgr. Jana Vašíčková, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX - Faculty of Science
Contact Person: prof. RNDr. Jakub Hofman, Ph.D.
NOW ( Bi7533 Soil ecotoxicology )
It is good if students attend Bi7533 Soil ecotoxicology lecture as well. Basic knowledge of general ecotoxicology is preferred.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15, only registered with preference (fields directly associated with the programme): 0/15
fields of study / plans the course is directly associated with
Course objectives
After attending the course, students should be able to:
Prepare OECD artificial soil and determine its properties
Calculate concentrations of tested chemical spiked to the soil
Prepare contaminated soil
Manipulate with soil organisms
Test soil ecotoxicity with four soil bioassays
Evaluate dose - response curves with several statistical methods
  • The course is laboratory praxis, where students are acquainted with basic soil ecotoxicity bioassays and learn how to perform these tests.
  • Steps:
  • 1. Start of earthworm Eisenia fetida culture
  • 2. Start of enchytraeid Enchytraeus albidus and Enchytraeus crypticus culture
  • 3. Start of springtail Folsomia candida culture
  • 4. Start of nematode Caenorhabditis elegans culture
  • 5. Learning how to care for cultures, culture books, plan of rota
  • 6. Care for cultures (routine during the term)
  • 7. Artificial soil preparation, control of pH and WHC
  • 8. Spiking the soil with toxic chemical at desired concentrations
  • 9. Start of Eisenia fetida test
  • 10. Start of Enchytraeus albidus and Enchytraeus crypticus tests
  • 11. Start of Folsomia candida test
  • 12. Start of Caenorhabditis elegans test and the evaluation
  • 13. Evaluation the results of Enchytraeus crypticus test
  • 14. Evaluation the results of Folsomia candida test
  • 15. Evaluation the results of Enchytraeus albidus test
  • 16. Evaluation the results of Eisenia fetida test
  • 17. Evaluation of dose - response curves - LC50, NOEC, LOEC a EC50
  • LOKKE, H. AND VAN GESTEL, C.A.M. (1998): Handbook of soil invertebrate toxicity tests. John Wiley & Sons, Chichester. ISBN 0-471-97103-0. 281p.
  • Soil ecotoxicology. Edited by Joseph Tarradellas - Gabriel Bitton - Dominique Rossel. Boca Raton: Lewis Publishers, 1996. 386 s. ISBN 1-56670-134-1. info
Teaching methods
At the beginning of the term, there are demonstration lectures followed by almost independent laboratory experiments performed in groups of students controlled and supervised by lecturer. Attendance in experimental work is mandatory. Work in the laboratory follows exact rules (manual to exercises) given by lecturer at the course beginning. Students work in teams and generate together also final reports.
Assessment methods
For credits it is mandatory: 1) be present in the course, 2) be activelly participating during the laboratory work, 3) provide final protocols (reports) containing overview of the methods used, gained results (measured and calculated indices), interpretation of the results.
Language of instruction
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Information on the extent and intensity of the course: bloková nepravidelná výuka cca 40 hod za semestr.
Teacher's information
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Spring 2008, Spring 2009, Spring 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2010, recent)
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