C7350 Protein characterisation using mass spectrometry - practice

Faculty of Science
Autumn 2014
Extent and Intensity
0/1/0. 1 credit(s) (plus extra credits for completion). Recommended Type of Completion: z (credit). Other types of completion: graded credit.
Teacher(s)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research - Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: National Centre for Biomolecular Research - Faculty of Science
Timetable
Fri 8:00–9:50 C02/211
Prerequisites (in Czech)
NOW ( C7250 Mass spectrometry of proteins )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to: understand basics of protein identification based on MS a MS/MS data;
select and use correct search software for identification of proteins;
understand basics of interpretation of MS/MS spectra;
Syllabus
  • Interpretation of MS data;
  • Interpretation of MALDI-MS/MS and ESI-MS/MS data;
  • Protein identification based on MS and MS/MS data;
  • Introduction into de novo sequencing;
  • Characterisation of protein modification - determination of type and site;
Literature
    recommended literature
  • SNYDER, Peter A. Interpreting protein mass spectra :a comprehensive resource. Oxford: Oxford University Press, 2000. xi, 522 s. ISBN 0-8412-3571-6. info
Teaching methods
practical work with data using PC
Assessment methods
individual processing of test data
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2013, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021.
  • Enrolment Statistics (Autumn 2014, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2014/C7350