FD010 Priciples of modern optics imaging methods

Faculty of Science
Autumn 2019
Extent and Intensity
1/1/0. 2 credit(s). Type of Completion: z (credit).
Mgr. Mojmír Meduňa, Ph.D. (lecturer)
prof. Mgr. Dominik Munzar, Dr. (lecturer)
Guaranteed by
prof. Mgr. Dominik Munzar, Dr.
Department of Condensed Matter Physics - Physics Section - Faculty of Science
Contact Person: Mgr. Mojmír Meduňa, Ph.D.
Supplier department: Department of Condensed Matter Physics - Physics Section - Faculty of Science
Mon 10:00–10:50 Kontaktujte učitele
  • Timetable of Seminar Groups:
FD010/01: Mon 11:00–11:50 Kontaktujte učitele
knowledge on optics and mathematics, both at the secondary school level
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The main objective of the course is to provide the students with the ability to
- list basic techniques of optical microscopy, imaging and explain the physical principles of these techniques
- apply the correct technique for a particular sample and examine this choice in terms of the obtained image contrast,
all for the micro structured samples in biology, medicine, mineralogy, metallurgy, material sciences etc.
Learning outcomes
Student will be able:
- understand the principles of basic optical devices;
- form a scheme of a given optical apparatus and to determine the actual path of light after the pass through;
- to list and understand the basic techniques of optical contrast in microscopes;
- to list some types of modern microscopes;
  • 1. Spectral composition of light, light absorption, light detectors, human eye 2. Beam splitters, reflectivity and transmissivity, total reflection, beam divergence 3. Optical imaging, thin lens, optical system, main planes, focal planes 4. Construction beams, real imaging beams, entrance diaphragm, angular aperture 5. Optical microscope, coherent and noncoherent illumination of sample, field lens 6. Limiting resolution, depth of field 7. Sample properties, absorption, double refraction, scattering, reflectance 8. Method of dark field in transmission and reflection 9. Double beam interference, time and spatial coherence, visibility 10. Imaging contrast in transmission and reflection in interference microscope. 11. Linear polarization of light, polarizer, transmission of light in double refraction materials 12. Polarization microscope, molecular structure of samples and double refraction. 13. Imaging of 3D samples, contrast by scanning methods, optical tomography 14. Principles of scanning computer optical microscopy methods
    recommended literature
  • KUBĚNA, Josef. Úvod do optiky. Brno: Masarykova univerzita, 1994. 181 s. ISBN 8021008350. info
  • SCHRÖDER, Gottfried. Technická optika : Technische Optik (Orig.). Translated by Zdeněk Berger. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1981. 158 s. info
  • Moderní optické zobrazovací metody - učební text J. Kuběna - na internetu: http://physics.muni.cz/~kubena/
Teaching methods
Integral part of this course is the excersize during which the topics from the lectures are worked through. The content of the excersize is formed by examples of the basic ray construction for the selected imaging aparatuses and/or methods.
Assessment methods
For successful completition of the course, passing the final written test is necessary. Unless stated otherwise, 80% attendance at the exercises is mandatory.
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020.
  • Enrolment Statistics (Autumn 2019, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2019/FD010