S2012 Applying Mendel’s laws of genetics to plant biology in the lab

Přírodovědecká fakulta
podzim 2019
Rozsah
1/2/0. 3 kr. (plus ukončení). Doporučované ukončení: zk. Jiná možná ukončení: k.
Vyučující
Helene Robert Boisivon, Ph.D. (přednášející)
Shekoufeh Ebrahimi Naghani, MSc (cvičící)
doc. Mgr. Jan Havliš, Dr. (pomocník)
Garance
Helene Robert Boisivon, Ph.D.
Národní centrum pro výzkum biomolekul - Přírodovědecká fakulta
Kontaktní osoba: Helene Robert Boisivon, Ph.D.
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul - Přírodovědecká fakulta
Rozvrh
St 13:00–13:50 A26-222, St 14:00–15:50 A26-222
Předpoklady
During the course all necessary information will be provided. However, basic knowledge of genetics is welcome.
Omezení zápisu do předmětu
Předmět je otevřen studentům libovolného oboru.
Předmět si smí zapsat nejvýše 10 stud.
Momentální stav registrace a zápisu: zapsáno: 1/10, pouze zareg.: 0/10, pouze zareg. s předností (mateřské obory): 0/10
Cíle předmětu
This course will use various laboratory techniques to test the segregation law of genetics as established by G. Mendel, the correlation between genotype and phenotype, and the effects of plant hormones for plant development. The lectures are accompanied by practical work.
Výstupy z učení
Students should be able to understand the analysis of segregation for a single genetic character, and the link between genotype and phenotype. Students will learn basic lab techniques. Students will be initiated to the role of plant hormones in plant development.
Osnova
  • Using Arabidopsis mutants the students will observe and calculate the frequencies of the mutant’s phenotypes, and correlate it with the genotype of individual seedlings. This part of the practical uses laboratory techniques such as work in sterile conditions, sow seeds for in vitro culture, monitoring seedling growth and analysis of the seedling phenotypes by stereomicroscope.
  • In a second part of the course, the student will investigate the effects of the plant hormone auxin on root growth (few concentrations tested) and on reporter lines for the hormone signalling pathway. Activation or repression of the signalling pathways will be tested by colorimetric staining. Results will be collected and conclusion will be written and discussed. This part of the practical includes tissue culture as in the first part (including preparation of the growth medium), result analysis (root growth measurements), colorimetric staining and microscopy.
Výukové metody
Lectures, exercises and practical work.
Metody hodnocení
Test and practical report.
Evaluation of the course is based on short exercises and practical report.
Vyučovací jazyk
Angličtina
Informace učitele
Planned schedule
(1) 2/10 – theory – part 1 - duration 2 h
Time: 13:00-15:30
Location: A26, 2.22

(2) 9/10 – Practical – 30 min lectures; 2h practical (sowing seeds) - duration   2 h 30 min
Time: 13:00-15:30
Location: A26, 2.22

(3) 14/10 - practical (counting) - duration 1 h
Time: 14:00-15:00
Location: A26

(4) 16/10 - practical (counting) - duration 1 h
Time: 14:00-15:00
Location: A26

(5) 17/10 - practical (counting) - duration 1 h
Time: 14:00-15:00
Location: A26

(6) 18/10 - practical (counting) - duration 1 h
Time: 14:00-15:00
Location: A26

(7) 23/10 - practical (data analysis) - duration 2 h
Time: 13:00-15:00
Location: A26, 2.22

(8) 30/10 - Theory part 2 - duration 2 h 30 min
Time: 13:00-15:30
Location: A26, 2.22

(9) 6/11 - Practical – 30 min lectures; 2h practical (preparation plates – sowing) - duration 2 to 4 h
Time: 13:00-17:00
Location: A26, 2.22

(10) 11/11 - practical (scans) - duration 1 h
Time: 14:00-15:00
Location: A26

(11) 15/11 - practical (scans) - duration 1 h
Time: 14:00-15:00
Location: A26

(12) 18/11 - practical (scans) - duration 1 h
Time: 14:00-15:00
Location: A26

(13) 22/11 - practical (scans + GUS staining) - duration 2 to 4 h
Time: 9:00-17:00 – 2 groups (morning and afternoon) depending on the number of students
Location: A26

(14) 25/11 - practical (microscopes)
Time: 9:00-17:00 – 2 groups (morning and afternoon) depending on the number of students
Location: A26

(15) 27/11 - Result analysis and discussion
Time: 13:00-15:00
Location: A26, 2.22

(16) 16/12 - Handling the reports
Další komentáře
Studijní materiály
Předmět je dovoleno ukončit i mimo zkouškové období.
Předmět je vyučován každoročně.
Předmět je zařazen také v obdobích podzim 2020.