Bi5880c Advanced methods in induced chlorophyll fluorescence - practice

Faculty of Science
Autumn 2023
Extent and Intensity
0/2. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
prof. Ing. Miloš Barták, CSc. (seminar tutor)
Mgr. Peter Váczi, Ph.D. (seminar tutor)
Guaranteed by
prof. Ing. Miloš Barták, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. Ing. Miloš Barták, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 15:00–16:50 C13/107a
Prerequisites
Bi4060 Plant physiology || SOUHLAS
A student interest in advanced methods in chlorophyll fluorescence technique
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
Practical exercises on chlorophyll fluorescence measuring devices. At the end of the course students will be able to choose a suitable method for measuring it and practically implement the selected plant material.
Learning outcomes
At the end of the course students will be able to choose a suitable method for measurement on plant material. The student will be able to apply the method properly.
Syllabus
  • Practical exercises using modern techniques aimed at measuring task, the focus is pozníní and practice the following areas of application-induced chlorophyll fluorescence in vivo: (1) Basic fluorescence ratio as an indicator of stress in PS II (2) Effective quantum yield of photochemical processes in PS II (3) Inductive effect: fast kinetics of chlorophyll fluorescnce (4) Inductive effect: Slow (Kautsky) kinetita chlorophyll fluorescence (5) and photochemical quenching of chlorophyll fluorescence nefotochemické (6) Energy quenching of chlorophyll fluorescence (7) Fotoinhibiční quenching of chlorophyll fluorescence (8) Imaging fluorescecnce chlorophyll (Chl fluorescence imaging) (9) Indication of the impact of blocking electron transport thalakoidní chloroplast membrane (10) Indication of the impact of toxic substances on the function of photosystem II (11) Indication of the impact of sub-optimal temperatures supra-function on PS II (12) Indication fotoinhibice influence on the function of PS II
Literature
    recommended literature
  • ROHÁČEK, Karel, Julie SOUKUPOVÁ and Miloš BARTÁK. Chlorophyll fluorescence: A wonderful tool to study plant physiology and plant stress. In Plant Cell Compartments - Selected Topics. Kerala - India: Research Signpost, 2008, p. 41-104. B. Schoefs (ed.), 1st edition. ISBN 978-81-308-0104-9. Research Signpost editorial web site info
  • ROHÁČEK, Karel and Miloš BARTÁK. Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. Photosynthetica. Praha: Elsevier, 1999, vol. 37, No 3, p. 339-363. ISSN 0300-3604. info
Teaching methods
Practical exercises using the method of "hands on" and simultaneous creation of measurement protocol.
Assessment methods
bezchybné protokoly, alespoň 70 % úspěšnost testu
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: Možno zadat studentovi / -ce projekt individuálně po dohodě s přednášejícím a cvičícím.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2023/Bi5880c