The effect of methanol and ionic strength on the reduction
process of 6-benzylaminopurine

D 2012

The effect of methanol and ionic strength on the reduction process of 6-benzylaminopurine

PILAŘOVÁ, Iveta; Přemysl LUBAL a Libuše TRNKOVÁ

Základní údaje

Originální název

The effect of methanol and ionic strength on the reduction process of 6-benzylaminopurine

Autoři

PILAŘOVÁ, Iveta; Přemysl LUBAL a Libuše TRNKOVÁ

Vydání

Praha, The 63rd Annual Meeting of the International Society of Electrochemistry - the book of abstracts, 1 s. 2012

Nakladatel

International Society of Electrochemistry

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10403 Physical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

6-benzylaminopurine;6-BAP; HMDE;EVLS;elimination voltammetry with linear scan;LSV;linear sweep voltammetry;methanol;

Změněno: 9. 1. 2013 11:05, Mgr. Sylvie Dohnalíková

Anotace

V originále

The reduction process of 6-benzylaminopurine (6-BAP), known as an adenine type of cytokinin, was investigated on mercury electrode by DC polarography [1, 2], cyclic and differential pulse voltammetry [2]. The aim of our contribution was the study of the effect of methanol content (1-20% v/v) and ionic strength (0.1- 1 M NaCl) on the reduction behavior of 6-BAP on a hanging mercury drop electrode (HMDE). It was found that the reduction of 6-BAP on HMDE proceeds in a protonated form, and therefore the research was devoted also to the study of protonation equilibria using potentiometry. Potentiometric titration provided the determination of protonation constants (pKa). The increase of methanol concentration causes pKa decrease of 6-BAP, and a significant and non-linear influence of ionic strength on protonation equilibrium was observed. In the ionic strength range from 0.1 to 0.5 M pKa decreases, at I = 0.5 M pKa reaches a minimum, and above this ionic strength value a pKa increase was observed. Linear sweep voltammetry (LSV) in connection with elimination voltammetry with linear scan (EVLS) [3,4] allows to determine not only the reduction potentials of 6-BAP and the effect of the two above-mentioned parameters but also prediction of the mechanism of the 6-BAP reduction process. Voltammetric experiments were carried out in buffered solutions (pH 3.21) at scan rate range 100 - 800 mV/s. From the LSV and EVLS experiments it follows that the new process is indicated in more negative potentials. This new process, taking place in adsorbed state, is sensitively revealed by EVLS in the form of a peak–counterpeak signal. Finally, in dependence on ionic strength and methanol content the kinetic parameters of the 6-BAP reduction process were determined and discussed [5].

Návaznosti

GD106/09/H035, projekt VaV

Název: Víceúrovňový design pokrokových materiálů

Investor: Grantová agentura ČR, Víceúrovňový design pokrokových materiálů

Citovat

PILAŘOVÁ, Iveta; Přemysl LUBAL a Libuše TRNKOVÁ. The effect of methanol and ionic strength on the reduction process of 6-benzylaminopurine. In The 63rd Annual Meeting of the International Society of Electrochemistry - the book of abstracts. Praha: International Society of Electrochemistry, 2012, 1 s.

@inproceedings{1067495,
   author = {Pilařová, Iveta and Lubal, Přemysl and Trnková, Libuše},
   address = {Praha},
   booktitle = {The 63rd Annual Meeting of the International Society of Electrochemistry - the book of abstracts},
   keywords = {6-benzylaminopurine;6-BAP; HMDE;EVLS;elimination voltammetry with linear scan;LSV;linear sweep voltammetry;methanol;},
   language = {eng},
   location = {Praha},
   publisher = {International Society of Electrochemistry},
   title = {The effect of methanol and ionic strength on the reduction process of 6-benzylaminopurine},
   year = {2012}
}

TY  - CONF
ID  - 1067495
AU  - Pilařová, Iveta - Lubal, Přemysl - Trnková, Libuše
PY  - 2012
TI  - The effect of methanol and ionic strength on the reduction process of 6-benzylaminopurine
PB  - International Society of Electrochemistry
CY  - Praha
KW  - 6-benzylaminopurine;6-BAP
KW  - HMDE;EVLS;elimination voltammetry with linear scan;LSV;linear sweep voltammetry;methanol;
N2  - The reduction process of 6-benzylaminopurine (6-BAP), known as an adenine type of cytokinin, was investigated on mercury electrode by DC polarography [1, 2], cyclic and differential pulse voltammetry [2]. The aim of our contribution was the study of the effect of methanol content (1-20% v/v) and ionic strength (0.1- 1 M NaCl) on the reduction behavior of 6-BAP on a hanging mercury drop electrode (HMDE). It was found that the reduction of 6-BAP on HMDE proceeds in a protonated form, and therefore the research was devoted also to the study of protonation equilibria using potentiometry. Potentiometric titration provided the determination of protonation constants (pKa). The increase of methanol concentration causes pKa decrease of 6-BAP, and a significant and non-linear influence of ionic strength on protonation equilibrium was observed. In the ionic strength range from 0.1 to 0.5 M pKa decreases, at I = 0.5 M pKa reaches a minimum, and above this ionic strength value a pKa increase was observed. Linear sweep voltammetry (LSV) in connection with elimination voltammetry with linear scan (EVLS) [3,4] allows to determine not only the reduction potentials of 6-BAP and the effect of the two above-mentioned parameters but also prediction of the mechanism of the 6-BAP reduction process. Voltammetric experiments were carried out in buffered solutions (pH 3.21) at scan rate range 100 - 800 mV/s. From the LSV and EVLS experiments it follows that the new process is indicated in more negative potentials. This new process, taking place in adsorbed state, is sensitively revealed by EVLS in the form of a peak–counterpeak signal. Finally, in dependence on ionic strength and methanol content the kinetic parameters of the 6-BAP reduction process were determined and discussed [5].
ER  -

PILAŘOVÁ, Iveta; Přemysl LUBAL a Libuše TRNKOVÁ. The effect of methanol and ionic strength on the reduction process of 6-benzylaminopurine. In \textit{The 63rd Annual Meeting of the International Society of Electrochemistry - the book of abstracts}. Praha: International Society of Electrochemistry, 2012, 1 s.

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