| LANDA, Leoš, Michal JURAJDA a Alexandra ŠULCOVÁ. Altered dopamine D1 and D2 receptor mRNA expression in mesencephalon from mice exposed to repeated treatments with methamphetamine and cannabinoid CB1 agonist methanandamide. Neuroendocrinology Letters. Stockholm: MAGHIRA & MAAS PUBLICATIONS, 2012, roč. 33, č. 4, s. 446-452. ISSN 0172-780X. |
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@article{1068154,
author = {Landa, Leoš and Jurajda, Michal and Šulcová, Alexandra},
article_location = {Stockholm},
article_number = {4},
keywords = {behavioural sensitization; methamphetamine; methanandamide; D1 and D2 receptor mRNA expression; mouse mesencephalon},
language = {eng},
issn = {0172-780X},
journal = {Neuroendocrinology Letters},
title = {Altered dopamine D1 and D2 receptor mRNA expression in mesencephalon from mice exposed to repeated treatments with methamphetamine and cannabinoid CB1 agonist methanandamide},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22936253},
volume = {33},
year = {2012}
}
TY - JOUR
ID - 1068154
AU - Landa, Leoš - Jurajda, Michal - Šulcová, Alexandra
PY - 2012
TI - Altered dopamine D1 and D2 receptor mRNA expression in mesencephalon from mice exposed to repeated treatments with methamphetamine and cannabinoid CB1 agonist methanandamide
JF - Neuroendocrinology Letters
VL - 33
IS - 4
SP - 446-452
EP - 446-452
PB - MAGHIRA & MAAS PUBLICATIONS
SN - 0172780X
KW - behavioural sensitization
KW - methamphetamine
KW - methanandamide
KW - D1 and D2 receptor mRNA expression
KW - mouse mesencephalon
UR - http://www.ncbi.nlm.nih.gov/pubmed/22936253
L2 - http://www.ncbi.nlm.nih.gov/pubmed/22936253
N2 - OBJECTIVES: In our previous studies we found that both acute administration of CB1 receptor agonist methanandamide and repeated methanandamide pretreatment prior to methamphetamine challenge dose elicited increase in the CB1 receptor mRNA expression in the mouse mesencephalon. As a reciprocal cross-talk is reported between the cannabinoid CB1 and dopamine receptors, that are highly co-localized on brain neurones, we targeted possible changes in relative expression of dopamine D1 and D2 receptor mRNA in mesencephalon in mice sensitized by repeated treatments to methamphetamine stimulatory effects and cross-sensitized to methamphetamine by cannabinoid CB1 receptor agonist methanandamide pre-treatment. METHODS: To confirm development of behavioural sensitization or cross-sensitization, respectively, we observed changes in locomotion using the open field test. Mice were treated repeatedly with either methamphetamine or methamphetamine after repeated pre-treatment with methanandamide. After each measurement of locomotion one third of animals were sacrificed and the brain was stored. RNA was isolated from the midbrain and used for reverse transcription and subsequent real-time PCR. RESULTS AND CONCLUSION: As in many of our earlier studies with the same dosage regimen we found in the behavioural part both development of sensitization to methamphetamine stimulatory effects after repeated treatment and crosssensitization to them by pre-treatment with cannabinoid receptor CB1 agonist methanandamide. Real-time PCR analyses showed an increase in D1 receptor mRNA expression after the first dose of methamphetamine (that persisted also after the last dose of methamphetamine) and after the first dose of methanandamide (which also persisted after the methamphetamine challenge dose).
ER -
LANDA, Leoš, Michal JURAJDA a Alexandra ŠULCOVÁ. Altered dopamine D1 and D2 receptor mRNA expression in mesencephalon from mice exposed to repeated treatments with methamphetamine and cannabinoid CB1 agonist methanandamide. \textit{Neuroendocrinology Letters}. Stockholm: MAGHIRA \&{} MAAS PUBLICATIONS, 2012, roč.~33, č.~4, s.~446-452. ISSN~0172-780X.
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