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@proceedings{1069034,
author = {Masařík, Michal and Sochor, Jiří and RuttkayandNedecky, Branislav and Křížková, Soňa and Stiborová, Marie and Eckschlager, Tomáš and Adam, Vojtěch and Kizek, René},
booktitle = {15th International Symposium on Molecular Medicine},
keywords = {oxidative stress; carboplatin; cisplatin; oxaliplatin; doxorubicin; ellipticine; FRAP; DPPH; ABTS; tumour cell lines},
language = {eng},
title = {A determination of oxidative stress in tumour cells treated with cytostatics},
year = {2012}
}
TY - CONF
ID - 1069034
AU - Masařík, Michal - Sochor, Jiří - Ruttkay-Nedecky, Branislav - Křížková, Soňa - Stiborová, Marie - Eckschlager, Tomáš - Adam, Vojtěch - Kizek, René
PY - 2012
TI - A determination of oxidative stress in tumour cells treated with cytostatics
KW - oxidative stress
KW - carboplatin
KW - cisplatin
KW - oxaliplatin
KW - doxorubicin
KW - ellipticine
KW - FRAP
KW - DPPH
KW - ABTS
KW - tumour cell lines
N2 - Oxidative stress as a response to anticancer drugs is not sufficiently investigated and direct measurement of reactive oxygen species and oxidative stress markers in clinical medicine is still difficult. In our study, oxidative status was studied in model tumour cell lines derived from tumours of the prostate (PC-3, PNT1A, 22RV1). Cell lines were cultured with anticancer drugs carboplatin, cisplatin, oxaliplatin at concentrations of 0, 10, 30 , 50, 80, 100, 150, 180, 200 and 250 micromol/l and drugs as doxorubicin and ellipticine at concentrations of 0, 0.5, 1, 1.5, 2, 4, 6, 10, 12, 15 micromol/l. The rate of oxidative stress was determined by antioxidant activity (FRAP, DPPH and ABTS). Based on the results obtained from oxidative changes in the cells determined by FRAP and ABTS, it was found the lowest antioxidant activity as 0.06 mg of Trolox/l was in cell lines 22RV1 treated with carboplatin at a concentration of 100 micromol/l. PNT1A cells gave the lowest antioxidant activity of 0.35 mg of Trolox/l after exposure to cisplatin concentration of 100 micromol/l.
ER -
MASAŘÍK, Michal, Jiří SOCHOR, Branislav RUTTKAY-NEDECKY, Soňa KŘÍŽKOVÁ, Marie STIBOROVÁ, Tomáš ECKSCHLAGER, Vojtěch ADAM a René KIZEK. A determination of oxidative stress in tumour cells treated with cytostatics. In \textit{15th International Symposium on Molecular Medicine}. 2012. ISSN~1107-3756.
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