A determination of oxidative stress in tumour cells treated
with cytostatics

a 2012

A determination of oxidative stress in tumour cells treated with cytostatics

MASAŘÍK, Michal; Jiří SOCHOR; Branislav RUTTKAY-NEDECKY; Soňa KŘÍŽKOVÁ; Marie STIBOROVÁ et al.

Základní údaje

Originální název

A determination of oxidative stress in tumour cells treated with cytostatics

Autoři

MASAŘÍK, Michal; Jiří SOCHOR; Branislav RUTTKAY-NEDECKY; Soňa KŘÍŽKOVÁ; Marie STIBOROVÁ; Tomáš ECKSCHLAGER; Vojtěch ADAM a René KIZEK

Vydání

15th International Symposium on Molecular Medicine, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.957

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

ISSN

UT WoS

000310651600169

Klíčová slova anglicky

oxidative stress; carboplatin; cisplatin; oxaliplatin; doxorubicin; ellipticine; FRAP; DPPH; ABTS; tumour cell lines

Změněno: 17. 10. 2012 11:29, Mgr. Martina Raudenská, Ph.D.

Anotace

V originále

Oxidative stress as a response to anticancer drugs is not sufficiently investigated and direct measurement of reactive oxygen species and oxidative stress markers in clinical medicine is still difficult. In our study, oxidative status was studied in model tumour cell lines derived from tumours of the prostate (PC-3, PNT1A, 22RV1). Cell lines were cultured with anticancer drugs carboplatin, cisplatin, oxaliplatin at concentrations of 0, 10, 30 , 50, 80, 100, 150, 180, 200 and 250 micromol/l and drugs as doxorubicin and ellipticine at concentrations of 0, 0.5, 1, 1.5, 2, 4, 6, 10, 12, 15 micromol/l. The rate of oxidative stress was determined by antioxidant activity (FRAP, DPPH and ABTS). Based on the results obtained from oxidative changes in the cells determined by FRAP and ABTS, it was found the lowest antioxidant activity as 0.06 mg of Trolox/l was in cell lines 22RV1 treated with carboplatin at a concentration of 100 micromol/l. PNT1A cells gave the lowest antioxidant activity of 0.35 mg of Trolox/l after exposure to cisplatin concentration of 100 micromol/l.

Citovat

MASAŘÍK, Michal; Jiří SOCHOR; Branislav RUTTKAY-NEDECKY; Soňa KŘÍŽKOVÁ; Marie STIBOROVÁ; Tomáš ECKSCHLAGER; Vojtěch ADAM a René KIZEK. A determination of oxidative stress in tumour cells treated with cytostatics. In 15th International Symposium on Molecular Medicine. 2012. ISSN 1107-3756.

@proceedings{1069034,
   author = {Masařík, Michal and Sochor, Jiří and RuttkayandNedecky, Branislav and Křížková, Soňa and Stiborová, Marie and Eckschlager, Tomáš and Adam, Vojtěch and Kizek, René},
   booktitle = {15th International Symposium on Molecular Medicine},
   keywords = {oxidative stress; carboplatin; cisplatin; oxaliplatin; doxorubicin; ellipticine; FRAP; DPPH; ABTS; tumour cell lines},
   language = {eng},
   title = {A determination of oxidative stress in tumour cells treated with cytostatics},
   year = {2012}
}

TY  - CONF
ID  - 1069034
AU  - Masařík, Michal - Sochor, Jiří - Ruttkay-Nedecky, Branislav - Křížková, Soňa - Stiborová, Marie - Eckschlager, Tomáš - Adam, Vojtěch - Kizek, René
PY  - 2012
TI  - A determination of oxidative stress in tumour cells treated with cytostatics
KW  - oxidative stress
KW  - carboplatin
KW  - cisplatin
KW  - oxaliplatin
KW  - doxorubicin
KW  - ellipticine
KW  - FRAP
KW  - DPPH
KW  - ABTS
KW  - tumour cell lines
N2  - Oxidative stress as a response to anticancer drugs is not sufficiently investigated and direct measurement of reactive oxygen species and oxidative stress markers in clinical medicine is still difficult. In our study, oxidative status was studied in model tumour cell lines derived from tumours of the prostate (PC-3, PNT1A, 22RV1). Cell lines were cultured with anticancer drugs carboplatin, cisplatin, oxaliplatin at concentrations of 0, 10, 30 , 50, 80, 100, 150, 180, 200 and 250 micromol/l and drugs as doxorubicin and ellipticine at concentrations of 0, 0.5, 1, 1.5, 2, 4, 6, 10, 12, 15 micromol/l. The rate of oxidative stress was determined by antioxidant activity (FRAP, DPPH and ABTS). Based on the results obtained from oxidative changes in the cells determined by FRAP and ABTS, it was found the lowest antioxidant activity as 0.06 mg of Trolox/l was in cell lines 22RV1 treated with carboplatin at a concentration of 100 micromol/l. PNT1A cells gave the lowest antioxidant activity of 0.35 mg of Trolox/l after exposure to cisplatin concentration of 100 micromol/l.
ER  -

MASAŘÍK, Michal; Jiří SOCHOR; Branislav RUTTKAY-NEDECKY; Soňa KŘÍŽKOVÁ; Marie STIBOROVÁ; Tomáš ECKSCHLAGER; Vojtěch ADAM a René KIZEK. A determination of oxidative stress in tumour cells treated with cytostatics. In \textit{15th International Symposium on Molecular Medicine}. 2012. ISSN~1107-3756.

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