Expression of Bcl-2 and other regulatory genes in tumour
prostate cell lines

a 2012

Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines

MASAŘÍK, Michal; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ; Jaromír GUMULEC; Monika HOLUBOVÁ et al.

Základní údaje

Originální název

Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines

Autoři

Vydání

17th World Congress on Advances in Oncology, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.957

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

ISSN

UT WoS

000310651600171

Klíčová slova anglicky

Bcl-2; p53; c-Fos; c-Jun; Ki-67; prostate cancer; cell lines; qRT-PCR

Změněno: 14. 2. 2013 15:14, doc. MUDr. Jaromír Gumulec, Ph.D.

Anotace

V originále

Prostate cancer is the second most frequently diagnosed cancer. Due to its high incidence and mortality, early diagnosis, identification of highly aggressive forms of clinically silent and understanding of disease pathogenesis with typical metabolic differences in order to develop specifically targeted therapy are needed. There have been found several compounds connected with tumourigenesis in prostate cells including Bcl-2. Bcl-2 acts as an inhibitor of apoptosis. It has been suggested that overexpression of Bcl-2 in human cancer cells contributes to their resistance to chemotherapy- and radiotherapy-induced apoptosis and is connected with unfavourable prognosis. In the fact, the majority of human prostate tumours overexpress Bcl-2. Possible associations with other proteins connected with tumour processes as c-Fos, c-Jun, Ki-67, NF-KB and p53 can be assumed. Therefore, we aimed our attention on determining of expression of Bcl-2, c-Fos, c-Jun, Ki-67,NF-KB and p53 genes in two prostate cell lines as 22Rvl cell line, a model of aggressive partially androgen-sensitive prostate cancer and PNTlA cell line, a model of healthy cell line. Moreover, we were interested in the issue how exposure of these cell lines to zinc(Il) ions could influence expression of the above mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation andhigh expression of Bcl-2.

Citovat

MASAŘÍK, Michal; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ; Jaromír GUMULEC; Monika HOLUBOVÁ; Jan BALVAN; Marián HLAVNA; Veronika TANHÄUSEROVÁ; Petr BABULA; Soňa KŘÍŽKOVÁ; Vojtěch ADAM a René KIZEK. Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines. In 17th World Congress on Advances in Oncology. 2012. ISSN 1107-3756.

@proceedings{1069043,
   author = {Masařík, Michal and Sztalmachová, Markéta and Raudenská, Martina and Gumulec, Jaromír and Holubová, Monika and Balvan, Jan and Hlavna, Marián and Tanhäuserová, Veronika and Babula, Petr and Křížková, Soňa and Adam, Vojtěch and Kizek, René},
   booktitle = {17th World Congress on Advances in Oncology},
   keywords = {Bcl-2; p53; c-Fos; c-Jun; Ki-67; prostate cancer; cell lines; qRT-PCR},
   language = {eng},
   title = {Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines},
   year = {2012}
}

TY  - CONF
ID  - 1069043
AU  - Masařík, Michal - Sztalmachová, Markéta - Raudenská, Martina - Gumulec, Jaromír - Holubová, Monika - Balvan, Jan - Hlavna, Marián - Tanhäuserová, Veronika - Babula, Petr - Křížková, Soňa - Adam, Vojtěch - Kizek, René
PY  - 2012
TI  - Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines
KW  - Bcl-2
KW  - p53
KW  - c-Fos
KW  - c-Jun
KW  - Ki-67
KW  - prostate cancer
KW  - cell lines
KW  - qRT-PCR
N2  - Prostate cancer is the second most frequently diagnosed cancer. Due to its high incidence and mortality, early diagnosis, identification of highly aggressive forms of clinically silent and understanding of disease pathogenesis with typical metabolic differences in order to develop specifically targeted therapy are needed. There have been found several compounds connected with tumourigenesis in prostate cells including Bcl-2. Bcl-2 acts as an inhibitor of apoptosis. It has been suggested that overexpression of Bcl-2 in human cancer cells contributes to their resistance to chemotherapy- and radiotherapy-induced apoptosis and is connected with unfavourable prognosis. In the fact, the majority of human prostate tumours overexpress Bcl-2. Possible associations with other proteins connected with tumour processes as c-Fos, c-Jun, Ki-67, NF-KB and p53 can be assumed. Therefore, we aimed our attention on determining of expression of Bcl-2, c-Fos, c-Jun, Ki-67,NF-KB and p53 genes in two prostate cell lines as 22Rvl cell line, a model of aggressive partially androgen-sensitive prostate cancer and PNTlA cell line, a model of healthy cell line. Moreover, we were interested in the issue how exposure of these cell lines to zinc(Il) ions could influence expression of the above mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation andhigh expression of Bcl-2.
ER  -

MASAŘÍK, Michal; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ; Jaromír GUMULEC; Monika HOLUBOVÁ; Jan BALVAN; Marián HLAVNA; Veronika TANHÄUSEROVÁ; Petr BABULA; Soňa KŘÍŽKOVÁ; Vojtěch ADAM a René KIZEK. Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines. In \textit{17th World Congress on Advances in Oncology}. 2012. ISSN~1107-3756.

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