ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM
PROSTATE CARCINOMA

a 2012

ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM PROSTATE CARCINOMA

HUDCOVÁ, Kristýna, Martina RAUDENSKÁ, Marián HLAVNA, Jaromír GUMULEC, Markéta SZTALMACHOVÁ et. al.

Základní údaje

Originální název

ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM PROSTATE CARCINOMA

Autoři

HUDCOVÁ, Kristýna, Martina RAUDENSKÁ, Marián HLAVNA, Jaromír GUMULEC, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Petr BABULA, Vojtěch ADAM, René KIZEK a Michal MASAŘÍK

Vydání

12th Workshop of Physical Chemists and Electrochemists, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.729 v roce 2011

ISBN

978-80-7375-618-5

ISSN

Klíčová slova česky

miRNA; prostate carcinoma; qRT-PCR

Změněno: 29. 1. 2016 13:35, doc. MUDr. Jaromír Gumulec, Ph.D.

Anotace

V originále

MiRNAs constitute a large class of single-stranded RNA molecules of 19 to 25 nucleotides that are involved in post-transcriptional gene silencing. MiRNAs not only regulate various developmental and physiologic processes but also are involved in cancer development and can play a role as an important biomarkers. The aim of this study was to determinate the expression levels of selected miRNA in the non-tumor PNT1A line in comparison with tumor lines 22Rv1, PC-3, and LNCaP. We used the TaqMan Gene Expression Assays and quantitative RT-PCR. We observed significantly higher (p<0.01) expression of miRNA 23a in all tumor lines compared to non-tumor PNT1A. Significantly higher (p<0.01) expression of miRNA 224 in 22Rv1 line compared to others and significantly higher (p<0.01) expression of miRNA 375 in all tumor lines in comparison with non-tumor PNT1A.

Citovat

HUDCOVÁ, Kristýna, Martina RAUDENSKÁ, Marián HLAVNA, Jaromír GUMULEC, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Petr BABULA, Vojtěch ADAM, René KIZEK a Michal MASAŘÍK. ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM PROSTATE CARCINOMA. In 12th Workshop of Physical Chemists and Electrochemists. 2012. ISBN 978-80-7375-618-5.

@proceedings{1070199,
   author = {Hudcová, Kristýna and Raudenská, Martina and Hlavna, Marián and Gumulec, Jaromír and Sztalmachová, Markéta and Tanhäuserová, Veronika and Babula, Petr and Adam, Vojtěch and Kizek, René and Masařík, Michal},
   booktitle = {12th Workshop of Physical Chemists and Electrochemists},
   language = {eng},
   isbn = {978-80-7375-618-5},
   title = {ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM PROSTATE CARCINOMA},
   year = {2012}
}

TY  - CONF
ID  - 1070199
AU  - Hudcová, Kristýna - Raudenská, Martina - Hlavna, Marián - Gumulec, Jaromír - Sztalmachová, Markéta - Tanhäuserová, Veronika - Babula, Petr - Adam, Vojtěch - Kizek, René - Masařík, Michal
PY  - 2012
TI  - ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM PROSTATE CARCINOMA
SN  - 9788073756185
N2  - MiRNAs constitute a large class of single-stranded RNA molecules of 19 to 25 nucleotides that are involved in post-transcriptional gene silencing. MiRNAs not only regulate various developmental and physiologic processes but also are involved in cancer development and can play a role as an important biomarkers. The aim of this study was to determinate the expression levels of selected miRNA in the non-tumor PNT1A line in comparison with tumor lines 22Rv1, PC-3, and LNCaP. We used the TaqMan Gene Expression Assays and quantitative RT-PCR. We observed significantly higher (p<0.01) expression of miRNA 23a in all tumor lines compared to non-tumor PNT1A. Significantly higher (p<0.01) expression of miRNA 224 in 22Rv1 line compared to others and significantly higher (p<0.01) expression of miRNA 375 in all tumor lines in comparison with non-tumor PNT1A.
ER  -

HUDCOVÁ, Kristýna, Martina RAUDENSKÁ, Marián HLAVNA, Jaromír GUMULEC, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Petr BABULA, Vojtěch ADAM, René KIZEK a Michal MASAŘÍK. ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM PROSTATE CARCINOMA. In \textit{12th Workshop of Physical Chemists and Electrochemists}. 2012. ISBN~978-80-7375-618-5.

Podrobný výpis o publikaci