Cisplatin GG-crosslinks within single-stranded DNA: Origin of
the preference for left-handed helicity

J 2012

Cisplatin GG-crosslinks within single-stranded DNA: Origin of the preference for left-handed helicity

KOZELKA, Jiří a Jordan MONNET

Základní údaje

Originální název

Cisplatin GG-crosslinks within single-stranded DNA: Origin of the preference for left-handed helicity

Autoři

KOZELKA, Jiří a Jordan MONNET

Vydání

Journal of Inorganic Biochemistry, Elsevier, 2012, 0162-0134

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10404 Polymer science

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.197

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/12:00061914

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.jinorgbio.2012.05.015

UT WoS

000309990500014

Klíčová slova anglicky

Anticancer drugs; Cisplatin; DNA binding; Intrastrand crosslinks; Molecular dynamics simulations; NMR spectroscopy

Štítky

AKR, rivok

Změněno: 9. 4. 2013 14:55, Ing. Andrea Mikešková

Anotace

V originále

Molecular dynamics (MD) simulations of the single-stranded DNA trinucleotide TG*G*, with the G* guanines crosslinked by the antitumor drug cisplatin, were performed with explicit representation of the water as solvent. The purpose of the simulations was to explain previous NMR observations indicating that in singlestranded cisplatin-DNA adducts, the crosslinked guanines adopt a left-handed helical orientation, whereas in duplexes, the orientation is right handed. The analysis of the MD trajectory of TG*G* has ascribed a crucial role to hydrogen-bonding (direct or through water) interactions of the 5prime oriented NH3 ligand of platinum with acceptor groups at the 5prime-side of the crosslink, namely the TpG* phosphate and the terminal 5prime OH group. These interactions bring about some strain into the trinucleotide which is slightly but significantly (1 to 1.5 kcal/mol) higher for the right-handed orientation than for the left-handed one. During the unconstrained, 3 ns long MD simulation, left handed conformations were ~15 times more abundant than the right handed ones. This sampling difference agrees roughlywith the calculated energy difference in strain energy. Overall, these results show that the Pt GG crosslink within single stranded DNA ismalleable and can access different conformations at a moderate energy cost. This malleability could be of importance in interactions between the platinated DNA and cellular proteins, in which the DNA is locally unwound.

Citovat

KOZELKA, Jiří a Jordan MONNET. Cisplatin GG-crosslinks within single-stranded DNA: Origin of the preference for left-handed helicity. Journal of Inorganic Biochemistry. Elsevier, 2012, roč. 115, č. 1, s. 106-112. ISSN 0162-0134. Dostupné z: https://doi.org/10.1016/j.jinorgbio.2012.05.015.

@article{1073461,
   author = {Kozelka, Jiří and Monnet, Jordan},
   article_number = {1},
   doi = {https://doi.org/10.1016/j.jinorgbio.2012.05.015},
   keywords = {Anticancer drugs; Cisplatin; DNA binding; Intrastrand crosslinks; Molecular dynamics simulations; NMR spectroscopy},
   language = {eng},
   issn = {0162-0134},
   journal = {Journal of Inorganic Biochemistry},
   title = {Cisplatin GG-crosslinks within single-stranded DNA: Origin of the preference for left-handed helicity},
   volume = {115},
   year = {2012}
}

TY  - JOUR
ID  - 1073461
AU  - Kozelka, Jiří - Monnet, Jordan
PY  - 2012
TI  - Cisplatin GG-crosslinks within single-stranded DNA: Origin of the preference for left-handed helicity
JF  - Journal of Inorganic Biochemistry
VL  - 115
IS  - 1
SP  - 106-112
EP  - 106-112
PB  - Elsevier
SN  - 01620134
KW  - Anticancer drugs
KW  - Cisplatin
KW  - DNA binding
KW  - Intrastrand crosslinks
KW  - Molecular dynamics simulations
KW  - NMR spectroscopy
N2  - Molecular dynamics (MD) simulations of the single-stranded DNA trinucleotide TG*G*, with the G* guanines crosslinked by the antitumor drug cisplatin, were performed with explicit representation of the water as solvent. The purpose of the simulations was to explain previous NMR observations indicating that in singlestranded cisplatin-DNA adducts, the crosslinked guanines adopt a left-handed helical orientation, whereas in duplexes, the orientation is right handed. The analysis of the MD trajectory of TG*G* has ascribed a crucial role to hydrogen-bonding (direct or through water) interactions of the 5prime oriented NH3 ligand of platinum with acceptor groups at the 5prime-side of the crosslink, namely the TpG* phosphate and the terminal 5prime OH group. These interactions bring about some strain into the trinucleotide which is slightly but significantly (1 to 1.5 kcal/mol) higher for the right-handed orientation than for the left-handed one. During the unconstrained, 3 ns long MD simulation, left handed conformations were ~15 times more abundant than the right handed ones. This sampling difference agrees roughlywith the calculated energy difference in strain energy. Overall, these results show that the Pt GG crosslink within single stranded DNA ismalleable and can access different conformations at a moderate energy cost. This malleability could be of importance in interactions between the platinated DNA and cellular proteins, in which the DNA is locally unwound.
ER  -

KOZELKA, Jiří a Jordan MONNET. Cisplatin GG-crosslinks within single-stranded DNA: Origin of the preference for left-handed helicity. \textit{Journal of Inorganic Biochemistry}. Elsevier, 2012, roč.~115, č.~1, s.~106-112. ISSN~0162-0134. Dostupné z: https://doi.org/10.1016/j.jinorgbio.2012.05.015.

Přehled o publikaci