SH3b cell wall binding domain of bacteriophage 812 endolysin:
Cloning, expression and structure determination

a 2012

SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination

BENEŠÍK, Martin, Jiří NOVÁČEK, Lubomír JANDA, Radka DOPITOVÁ, Lukáš ŽÍDEK et. al.

Základní údaje

Originální název

SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination

Autoři

BENEŠÍK, Martin, Jiří NOVÁČEK, Lubomír JANDA, Radka DOPITOVÁ, Lukáš ŽÍDEK, Jiří DOŠKAŘ, Vladislava RŮŽIČKOVÁ, Marek MOŠA a Roman PANTŮČEK

Vydání

Viruses of Microbes. From exploration to applications in the -omics era. EMBO Conference Series, Brussels, Belgium, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Belgie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Staphylococcus; bacteriophage; Myoviridae; endolysin; SH3b domain; NMR

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 11. 2012 11:34, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Staphylococcus aureus is a major causative agent of human and animal diseases. The increasing number of pathogenic strains resistant to antimicrobial drugs is a serious public health problem that can be solved by applications of phage therapy as a suitable alternative to antibiotics treatment. Currently the research focus on the phage encoded antimicrobial proteins applicable to combat bacterial infections, such as phage endolysins that digest the cell wall of bacteria. In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of SPO1-like viruses from family Myoviridae. Preliminary results of the NMR analysis confirmed the presence of the well defined structure of the studied construct, and provided the sequential assignment of all resonance frequencies along the peptide backbone allowing us to assess the presence of the secondary structure motifs.

Návaznosti

TA01010405, projekt VaV

Název: Výzkum stafylokokových bakteriofágových mutant s širokým spektrem hostitelů (Akronym: TAČR/IMUNA-1)

Investor: Technologická agentura ČR, Výzkum stafylokokových bakteriofágových mutant s širokým spektrem hostitelů

Citovat

BENEŠÍK, Martin, Jiří NOVÁČEK, Lubomír JANDA, Radka DOPITOVÁ, Lukáš ŽÍDEK, Jiří DOŠKAŘ, Vladislava RŮŽIČKOVÁ, Marek MOŠA a Roman PANTŮČEK. SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination. In Viruses of Microbes. From exploration to applications in the -omics era. EMBO Conference Series, Brussels, Belgium. 2012.

@proceedings{1074370,
   author = {Benešík, Martin and Nováček, Jiří and Janda, Lubomír and Dopitová, Radka and Žídek, Lukáš and Doškař, Jiří and Růžičková, Vladislava and Moša, Marek and Pantůček, Roman},
   booktitle = {Viruses of Microbes. From exploration to applications in the -omics era. EMBO Conference Series, Brussels, Belgium},
   keywords = {Staphylococcus; bacteriophage; Myoviridae; endolysin; SH3b domain; NMR},
   language = {eng},
   title = {SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination},
   url = {http://events.embo.org/12-virus-microbe/},
   year = {2012}
}

TY  - CONF
ID  - 1074370
AU  - Benešík, Martin - Nováček, Jiří - Janda, Lubomír - Dopitová, Radka - Žídek, Lukáš - Doškař, Jiří - Růžičková, Vladislava - Moša, Marek - Pantůček, Roman
PY  - 2012
TI  - SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination
KW  - Staphylococcus
KW  - bacteriophage
KW  - Myoviridae
KW  - endolysin
KW  - SH3b domain
KW  - NMR
UR  - http://events.embo.org/12-virus-microbe/
N2  - Staphylococcus aureus is a major causative agent of human and animal diseases. The increasing number of pathogenic strains resistant to antimicrobial drugs is a serious public health problem that can be solved by applications of phage therapy as a suitable alternative to antibiotics treatment. Currently the research focus on the phage encoded antimicrobial proteins applicable to combat bacterial infections, such as phage endolysins that digest the cell wall of bacteria. In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of SPO1-like viruses from family Myoviridae. Preliminary results of the NMR analysis confirmed the presence of the well defined structure of the studied construct, and provided the sequential assignment of all resonance frequencies along the peptide backbone allowing us to assess the presence of the secondary structure motifs.
ER  -

BENEŠÍK, Martin, Jiří NOVÁČEK, Lubomír JANDA, Radka DOPITOVÁ, Lukáš ŽÍDEK, Jiří DOŠKAŘ, Vladislava RŮŽIČKOVÁ, Marek MOŠA a Roman PANTŮČEK. SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination. In \textit{Viruses of Microbes. From exploration to applications in the -omics era. EMBO Conference Series, Brussels, Belgium}. 2012.

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