IDENTIFICATION AND FUNCTIONAL ANALYSIS OF microRNAs WITH
ALTERED EXPRESSION IN COLORECTAL CANCER TISSUE

a 2012

IDENTIFICATION AND FUNCTIONAL ANALYSIS OF microRNAs WITH ALTERED EXPRESSION IN COLORECTAL CANCER TISSUE

FALTEJSKOVÁ, Petra, Marek SVOBODA, Klára ŠRŮTOVÁ, Jitka MLČOCHOVÁ, Rostislav VYZULA et. al.

Základní údaje

Originální název

IDENTIFICATION AND FUNCTIONAL ANALYSIS OF microRNAs WITH ALTERED EXPRESSION IN COLORECTAL CANCER TISSUE

Autoři

FALTEJSKOVÁ, Petra, Marek SVOBODA, Klára ŠRŮTOVÁ, Jitka MLČOCHOVÁ, Rostislav VYZULA a Ondřej SLABÝ

Vydání

Regulatory & Non-coding RNAs, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Masarykův onkologický ústav

Klíčová slova anglicky

microRNAs; oncology; colorectal cancer; DLD-1; HCT-116

Příznaky

Mezinárodní význam

Změněno: 28. 11. 2012 11:19, Mgr. Petra Vychytilová, Ph.D.

Anotace

V originále

MicroRNAs (miRNAs) constitute a robust regulatory network with posttranscriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumor suppressors. We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoral tissues and identified 42 differentially expressed miRNAs. We chose miR-215, miR-375, miR-378, miR-422a and miR-135b for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro analyses. MiR-215, miR-375, miR-378 and miR-422a were significantly decreased, whereas miR-135b was increased in CRC tumor tissues. Levels of miR-215 and miR-422a correlated with clinical stage. MiR-135b was associated with higher preoperative serum levels of CEA and CA19-9. In vitro analyses showed that ectopic expression of miR-215 decreases viability and migration, increases apoptosis and promotes cell cycle arrest in DLD-1 and HCT-116 colon cancer cell lines. Similarly, over-expression of miR-375 and inhibition of miR-135b led to decreased viability. Finally, restoration of miR-378, miR-422a and miR-375 inhibited G1/S transition. These findings indicate that miR-378, miR-375, miR-422a and miR-215 play an important role in CRC as tumor suppressors, whereas miR-135b functions as an oncogene; both groups of miRNA contribute to CRC pathogenesis.

Citovat

FALTEJSKOVÁ, Petra, Marek SVOBODA, Klára ŠRŮTOVÁ, Jitka MLČOCHOVÁ, Rostislav VYZULA a Ondřej SLABÝ. IDENTIFICATION AND FUNCTIONAL ANALYSIS OF microRNAs WITH ALTERED EXPRESSION IN COLORECTAL CANCER TISSUE. In Regulatory & Non-coding RNAs, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. 2012.

@proceedings{1075186,
   author = {Faltejsková, Petra and Svoboda, Marek and Šrůtová, Klára and Mlčochová, Jitka and Vyzula, Rostislav and Slabý, Ondřej},
   booktitle = {Regulatory & Non-coding RNAs, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York},
   keywords = {microRNAs; oncology; colorectal cancer; DLD-1; HCT-116},
   language = {eng},
   title = {IDENTIFICATION AND FUNCTIONAL ANALYSIS OF microRNAs WITH ALTERED EXPRESSION IN COLORECTAL CANCER TISSUE},
   year = {2012}
}

TY  - CONF
ID  - 1075186
AU  - Faltejsková, Petra - Svoboda, Marek - Šrůtová, Klára - Mlčochová, Jitka - Vyzula, Rostislav - Slabý, Ondřej
PY  - 2012
TI  - IDENTIFICATION AND FUNCTIONAL ANALYSIS OF microRNAs WITH ALTERED EXPRESSION IN COLORECTAL CANCER TISSUE
KW  - microRNAs
KW  - oncology
KW  - colorectal cancer
KW  - DLD-1
KW  - HCT-116
N2  - MicroRNAs (miRNAs) constitute a robust regulatory network with posttranscriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumor suppressors. We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoral tissues and identified 42 differentially expressed miRNAs. We chose miR-215, miR-375, miR-378, miR-422a and miR-135b for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro analyses. MiR-215, miR-375, miR-378 and miR-422a were significantly decreased, whereas miR-135b was increased in CRC tumor tissues. Levels of miR-215 and miR-422a correlated with clinical stage. MiR-135b was associated with higher preoperative serum levels of CEA and CA19-9. In vitro analyses showed that ectopic expression of miR-215 decreases viability and migration, increases apoptosis and promotes cell cycle arrest in DLD-1 and HCT-116 colon cancer cell lines. Similarly, over-expression of miR-375 and inhibition of miR-135b led to decreased viability. Finally, restoration of miR-378, miR-422a and miR-375 inhibited G1/S transition. These findings indicate that miR-378, miR-375, miR-422a and miR-215 play an important role in CRC as tumor suppressors, whereas miR-135b functions as an oncogene; both groups of miRNA contribute to CRC pathogenesis.
ER  -

FALTEJSKOVÁ, Petra, Marek SVOBODA, Klára ŠRŮTOVÁ, Jitka MLČOCHOVÁ, Rostislav VYZULA a Ondřej SLABÝ. IDENTIFICATION AND FUNCTIONAL ANALYSIS OF microRNAs WITH ALTERED EXPRESSION IN COLORECTAL CANCER TISSUE. In \textit{Regulatory \&{} Non-coding RNAs, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York}. 2012.

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