Sequencing-based Molecular Typing of Treponema pallidum Strains
in the Czech Republic: All Identified Genotypes are Related to
the Sequence of the SS14 Strain

J 2012

Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain

FLASAROVÁ, Magdalena, Petra POSPÍŠILOVÁ, Lenka MIKALOVÁ, Zuzana VALIŠOVÁ, Eliška DASTYCHOVÁ et. al.

Základní údaje

Originální název

Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain

Autoři

FLASAROVÁ, Magdalena (203 Česká republika, garant, domácí), Petra POSPÍŠILOVÁ (203 Česká republika, domácí), Lenka MIKALOVÁ (203 Česká republika, domácí), Zuzana VALIŠOVÁ (203 Česká republika, domácí), Eliška DASTYCHOVÁ (203 Česká republika, domácí), Radim STRNADEL (203 Česká republika), Ivana KUKLOVÁ (203 Česká republika), Vladana WOZNICOVÁ (203 Česká republika, domácí), Hana ZÁKOUCKÁ (203 Česká republika) a David ŠMAJS (203 Česká republika, domácí)

Vydání

Acta Dermato-Venereologica, 2012, 0001-5555

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.487

Kód RIV

RIV/00216224:14110/12:00057856

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.2340/00015555-1335

UT WoS

000310828300030

Klíčová slova anglicky

Treponema pallidum; syphilis; molecular typing; macrolide resistance; nested PCR

Příznaky

Mezinárodní význam

Změněno: 23. 4. 2013 00:55, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA was based on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes.

Návaznosti

GA310/07/0321, projekt VaV

Název: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Grantová agentura ČR, Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

NT11159, projekt VaV

Název: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministerstvo zdravotnictví ČR, Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Přiložené soubory

3664.pdf

Vyhledat podobné dokumenty

Citovat

FLASAROVÁ, Magdalena, Petra POSPÍŠILOVÁ, Lenka MIKALOVÁ, Zuzana VALIŠOVÁ, Eliška DASTYCHOVÁ, Radim STRNADEL, Ivana KUKLOVÁ, Vladana WOZNICOVÁ, Hana ZÁKOUCKÁ a David ŠMAJS. Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain. Acta Dermato-Venereologica. 2012, roč. 92, č. 6, s. 669–674. ISSN 0001-5555. Dostupné z: https://dx.doi.org/10.2340/00015555-1335.

@article{1075190,
   author = {Flasarová, Magdalena and Pospíšilová, Petra and Mikalová, Lenka and Vališová, Zuzana and Dastychová, Eliška and Strnadel, Radim and Kuklová, Ivana and Woznicová, Vladana and Zákoucká, Hana and Šmajs, David},
   article_number = {6},
   doi = {http://dx.doi.org/10.2340/00015555-1335},
   keywords = {Treponema pallidum; syphilis; molecular typing; macrolide resistance; nested PCR},
   language = {eng},
   issn = {0001-5555},
   journal = {Acta Dermato-Venereologica},
   title = {Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain},
   volume = {92},
   year = {2012}
}

TY  - JOUR
ID  - 1075190
AU  - Flasarová, Magdalena - Pospíšilová, Petra - Mikalová, Lenka - Vališová, Zuzana - Dastychová, Eliška - Strnadel, Radim - Kuklová, Ivana - Woznicová, Vladana - Zákoucká, Hana - Šmajs, David
PY  - 2012
TI  - Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain
JF  - Acta Dermato-Venereologica
VL  - 92
IS  - 6
SP  - 669–674
EP  - 669–674
SN  - 00015555
KW  - Treponema pallidum
KW  - syphilis
KW  - molecular typing
KW  - macrolide resistance
KW  - nested PCR
N2  - A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA was based on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes.
ER  -

FLASAROVÁ, Magdalena, Petra POSPÍŠILOVÁ, Lenka MIKALOVÁ, Zuzana VALIŠOVÁ, Eliška DASTYCHOVÁ, Radim STRNADEL, Ivana KUKLOVÁ, Vladana WOZNICOVÁ, Hana ZÁKOUCKÁ a David ŠMAJS. Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain. \textit{Acta Dermato-Venereologica}. 2012, roč.~92, č.~6, s.~669–674. ISSN~0001-5555. Dostupné z: https://dx.doi.org/10.2340/00015555-1335.

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