Bilateral activation of microglia and expression of TLR4 in the
trigeminal sbnucleus caudalis after unilateral infraorbital
nerve injury

a 2012

Bilateral activation of microglia and expression of TLR4 in the trigeminal sbnucleus caudalis after unilateral infraorbital nerve injury

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Základní údaje

Originální název

Bilateral activation of microglia and expression of TLR4 in the trigeminal sbnucleus caudalis after unilateral infraorbital nerve injury

Název česky

Bilaterální aktivace mikroglie a exprese TLR4 v trigemiálním subnucleus caudalis po jednostranném poškození nervus infraoritalis

Autoři

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Vydání

FENS Forum of Neuroscience Barcelona, 2012

Další údaje

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

mikroglie; TLR4; trigeminální subnucleus caudalis; chronická komprese n. infraorbitalis

Klíčová slova anglicky

microglia; TLR4; trigeminal subnucleus caudalis; chronic constriction of the infraorbital nerve

Příznaky

Mezinárodní význam

Změněno: 23. 12. 2013 15:27, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

The activated microglia contribute to increased excitation of neurons, neuropathic pain induction and maintenance. Microglia become activated after peripheral nerve injury in the spinal and trigeminal models of both neuropathic pain and produce various cytokines and inflammatory mediators. TLR4 is one of the pathogen recognition receptor which is expressed by glial cells in mediating neuroinflammatory response. The goal of our work was to investigate bilateral activation of microglia and expression of TLR4 in the trigeminal subnucleus caudalis (TSC) following unilateral chronic constriction injury of the infraorbital nerve (IONL) as model of neuropathic pain. The rats were anesthetized and the left ION was exposed and tightly ligated with 6-0 silk suture. The ION- and sham-operated rats were left to survive for 7 days. The naïve (n=3), ION- (n=3) and sham-operated (n=3) rats were perfused with Zamboni solution, brainstem was dissected, fixed overnight in the same fixative, and washed in 20% sucrose. Transverse cryostat sections were incubated with mouse monoclonal anti-OX42 antibody to identify microglia and/or simultaneously incubated with goat polyclonal antibody for TLR4. The corresponding secondary antibodies were conjugated with FITC and TRITC. The quantification was performed by image analysis system Lucia. An increased activation of microglia was observed bilaterally in the superficial lamina of TSC after unilateral IONL when compared with naïve TSC. The microglia activation was lower in contralateral than ipsilateral TSC. The TSC of sham-operated rats displayed also mild increased OX42 immunofluorescence. In contrast to OX42, TLR4 immunostaining was found only in microglia of ipsilateral TSC from rats operated on unilateral IONL. Bilateral activation of microglia in TSC following unilateral IONL indicates a propagation of neuroinflammatory reaction to contralateral side. In contrast, upregulation of TLR4 suggests rather direct reaction to neuron injury in ipsilateral TSC.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Bilateral activation of microglia and expression of TLR4 in the trigeminal sbnucleus caudalis after unilateral infraorbital nerve injury. In FENS Forum of Neuroscience Barcelona. 2012.

@proceedings{1075411,
   author = {Strejčková, Lucie and Klusáková, Ilona and Dubový, Petr},
   booktitle = {FENS Forum of Neuroscience Barcelona},
   keywords = {microglia; TLR4; trigeminal subnucleus caudalis; chronic constriction of the infraorbital nerve},
   title = {Bilateral activation of microglia and expression of TLR4 in the trigeminal sbnucleus caudalis after unilateral infraorbital nerve injury},
   url = {http://fens.ekonnect.co/FENS_331},
   year = {2012}
}

TY  - CONF
ID  - 1075411
AU  - Strejčková, Lucie - Klusáková, Ilona - Dubový, Petr
PY  - 2012
TI  - Bilateral activation of microglia and expression of TLR4 in the trigeminal sbnucleus caudalis after unilateral infraorbital nerve injury
KW  - microglia
KW  - TLR4
KW  - trigeminal subnucleus caudalis
KW  - chronic constriction of the infraorbital nerve
UR  - http://fens.ekonnect.co/FENS_331
N2  - The activated microglia contribute to increased excitation of neurons, neuropathic pain induction and maintenance. Microglia become activated after peripheral nerve injury in the spinal and trigeminal models of both neuropathic pain and produce various cytokines and inflammatory mediators. TLR4 is one of the pathogen recognition receptor which is expressed by glial cells in mediating neuroinflammatory response. The goal of our work was to investigate bilateral activation of microglia and expression of TLR4 in the trigeminal subnucleus caudalis (TSC) following unilateral chronic constriction injury of the infraorbital nerve (IONL) as model of neuropathic pain. The rats were anesthetized and the left ION was exposed and tightly ligated with 6-0 silk suture. The ION- and sham-operated rats were left to survive for 7 days. The naïve (n=3), ION- (n=3) and sham-operated (n=3) rats were perfused with Zamboni solution, brainstem was dissected, fixed overnight in the same fixative, and washed in 20% sucrose. Transverse cryostat sections were incubated with mouse monoclonal anti-OX42 antibody to identify microglia and/or simultaneously incubated with goat polyclonal antibody for TLR4. The corresponding secondary antibodies were conjugated with FITC and TRITC. The quantification was performed by image analysis system Lucia. An increased activation of microglia was observed bilaterally in the superficial lamina of TSC after unilateral IONL when compared with naïve TSC. The microglia activation was lower in contralateral than ipsilateral TSC. The TSC of sham-operated rats displayed also mild increased OX42 immunofluorescence. In contrast to OX42, TLR4 immunostaining was found only in microglia of ipsilateral TSC from rats operated on unilateral IONL. Bilateral activation of microglia in TSC following unilateral IONL indicates a propagation of neuroinflammatory reaction to contralateral side. In contrast, upregulation of TLR4 suggests rather direct reaction to neuron injury in ipsilateral TSC.
ER  -

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