Invasion of macrophages in trigeminal ganglion compartments not
related to injured nerve

a 2012

Invasion of macrophages in trigeminal ganglion compartments not related to injured nerve

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Základní údaje

Originální název

Invasion of macrophages in trigeminal ganglion compartments not related to injured nerve

Název česky

Invaze makrofágů v oblasti trigeminálního ganglia, které přímo nesouvisí s poškozením nervu

Autoři

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Vydání

Morphology 2012, 2012

Další údaje

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

makrofágy; komprese n. infraorbitalis; ganglion trigeminale

Klíčová slova anglicky

macrophages; constriction of infraorbital nerve; trigeminal ganglion

Změněno: 5. 2. 2013 10:35, MUDr. Ilona Klusáková, Ph.D.

Anotace

V originále

In context of peripheral nerve injury macrophages play a central role in Wallerian degeneration and produce many cytokines, which contribute to development and maintenance of neuropathic pain. The goal of our work was to investigate invasion of activated macrophages not only into the trigeminal ganglia (TG) compartments containing injured but also uninjured neurons in the model of neuropathic pain. For this purpose, unilateral chronic constriction injury of the infraorbital nerve (ION) was performed. The rats were anesthetized and the left ION was exposed and tightly ligated with 6-0 silk suture. Fluorogold (FG) was injected distal to ligated nerve to visualize neurons associated with ION. The ION- and sham-operated rats were left to survive for 3 and 7 days. The naïve (n=4), ION- (n=4) and sham-operated (n=4) rats were perfused with Zamboni solution and TG were dissected, fixed overnight, and washed in 20% sucrose. Longitudinal cryostat sections were incubated with rabbit polyclonal anti-ATF3 antibody to identify injured neurons and/or simultaneously incubated with mouse monoclonal antibody for ED-1. The corresponding secondary antibodies were conjugated with FITC and TRITC. The quantification of ED-1 positive cells was performed by image analysis system Lucia. Neuronal retrograde tracer (FG) visualized all TG neurons whose afferent axons contribute to ION, but ATF3 detected only injured neurons in maxillary compartment of ipsilateral TG. An increased invasion of activated ED-1+ macrophages was observed bilaterally in the TG after unilateral chronic constriction injury of the ION when compared with naïve ones. The number of ED-1+ macrophages was lower in contralateral than ipsilateral TG. The TG of sham-operated rats displayed also a mild bilateral invasion of ED-1+ cells. Although injured neurons of ION lie in the maxillary compartment, a moderate invasion of macrophages was detected also in the mandibular region of TG. Our results revealed that unilateral IONL induced invasion of activated macrophages not only into compartment containing injured (ATF3+), but also uninjured neurons of TG.

Návaznosti

CZ.1.05/1.1.00/02.0068, interní kód MU

Název: CEITEC - středoevropský technologický institut (Akronym: CEITEC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC - středoevropský technologický institut, 1.1 Evropská centra excelence

Citovat

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Invasion of macrophages in trigeminal ganglion compartments not related to injured nerve. In Morphology 2012. 2012.

@proceedings{1075414,
   author = {Strejčková, Lucie and Klusáková, Ilona and Dubový, Petr},
   booktitle = {Morphology 2012},
   keywords = {macrophages; constriction of infraorbital nerve; trigeminal ganglion},
   title = {Invasion of macrophages in trigeminal ganglion compartments not related to injured nerve},
   url = {http://www.lfhk.cuni.cz/anatomie/Morphology/main.html},
   year = {2012}
}

TY  - CONF
ID  - 1075414
AU  - Strejčková, Lucie - Klusáková, Ilona - Dubový, Petr
PY  - 2012
TI  - Invasion of macrophages in trigeminal ganglion compartments not related to injured nerve
KW  - macrophages
KW  - constriction of infraorbital nerve
KW  - trigeminal ganglion
UR  - http://www.lfhk.cuni.cz/anatomie/Morphology/main.html
N2  - In context of peripheral nerve injury macrophages play a central role in Wallerian degeneration and produce many cytokines, which contribute to development and maintenance of neuropathic pain. The goal of our work was to investigate invasion of activated macrophages not only into the trigeminal ganglia (TG) compartments containing injured but also uninjured neurons in the model of neuropathic pain. For this purpose, unilateral chronic constriction injury of the infraorbital nerve (ION) was performed. The rats were anesthetized and the left ION was exposed and tightly ligated with 6-0 silk suture. Fluorogold (FG) was injected distal to ligated nerve to visualize neurons associated with ION. The ION- and sham-operated rats were left to survive for 3 and 7 days. The naïve (n=4), ION- (n=4) and sham-operated (n=4) rats were perfused with Zamboni solution and TG were dissected, fixed overnight, and washed in 20% sucrose. Longitudinal cryostat sections were incubated with rabbit polyclonal anti-ATF3 antibody to identify injured neurons and/or simultaneously incubated with mouse monoclonal antibody for ED-1. The corresponding secondary antibodies were conjugated with FITC and TRITC. The quantification of ED-1 positive cells was performed by image analysis system Lucia. Neuronal retrograde tracer (FG) visualized all TG neurons whose afferent axons contribute to ION, but ATF3 detected only injured neurons in maxillary compartment of ipsilateral TG. An increased invasion of activated ED-1+ macrophages was observed bilaterally in the TG after unilateral chronic constriction injury of the ION when compared with naïve ones. The number of ED-1+ macrophages was lower in contralateral than ipsilateral TG. The TG of sham-operated rats displayed also a mild bilateral invasion of ED-1+ cells. Although injured neurons of ION lie in the maxillary compartment, a moderate invasion of macrophages was detected also in the mandibular region of TG. Our results revealed that unilateral IONL induced invasion of activated macrophages not only into compartment containing injured (ATF3+), but also uninjured neurons of TG.
ER  -

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Invasion of macrophages in trigeminal ganglion compartments not related to injured nerve. In \textit{Morphology 2012}. 2012.

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