Identification and functional analysis of microRNA deregulated
in colorectal cancer

a 2012

Identification and functional analysis of microRNA deregulated in colorectal cancer

FALTEJSKOVÁ, Petra; Jitka MLČOCHOVÁ; Klára ŠRŮTOVÁ; Andrej BEŠŠE; Lenka RADOVÁ et. al.

Základní údaje

Originální název

Identification and functional analysis of microRNA deregulated in colorectal cancer

Autoři

FALTEJSKOVÁ, Petra ; Jitka MLČOCHOVÁ; Klára ŠRŮTOVÁ; Andrej BEŠŠE; Lenka RADOVÁ; Marek SVOBODA; Rostislav VYZULA a Ondřej SLABÝ

Vydání

3rd ICCTI Workshop - MicroRNAs in Oncology, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14740/12:00062178

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

microRNAs; oncology; colorectal cancer; DLD-1; HCT-116

Změněno: 25. 4. 2013 10:36, Mgr. Petra Vychytilová, Ph.D.

Anotace

V originále

Colorectal cancer (CRC) is one of the most common types of cancers in the Czech Republic and it is the third leading cause of cancer-related death in the Western countries. The main reasons of high mortality are diagnosis at the stage of advanced disease, insufficient clinical classification and low level of therapy individualization associated with unsatisfactory therapy responses. That is the reason why several efforts have been made to find new biomarkers in CRC enabling early diagnosis of asymptomatic disease, accurate differentiation between particular clinical stages and individualization of the therapy. MicroRNAs (miRNAs) are small, non-coding, single-stranded RNAs, 18 25 nucleotides in length, that are endogenously expressed and post-transcriptionally regulate gene expression. There is an increasing evidence that miRNAs can function as both tumor suppressors and as oncogenes and therefore play an important role in regulation of many biological processes such as cell cycle, proliferation, differentiation, apoptosis or invasiveness. The aim of our study was to determine the expression profiles of 667 miRNAs in CRC tissues and paired non-tumoral tissues and identify miRNAs with deregulated expression in CRC. Selected miRNAs were further validated and their role in regulation of cell cycle, apoptosis, cell viability and metastasing was analyzed in vitro using transient transfection and stable cell lines derived from colorectal cancer. We suppose that miRNAs or proteins that are under their control could serve as potential diagnostic, prognostic and predictive biomarkers, which can be identified not only in tumor tissues, but also in serum of CRC patients. The results of our research will be presented.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

NT13549, projekt VaV

Název: Vytvoření diagnostické sady cirkulujících mikroRNA pro neinvazivní časnou diagnostiku a sledování pacientů s kolorektálním karcinomem

Citovat

FALTEJSKOVÁ, Petra; Jitka MLČOCHOVÁ; Klára ŠRŮTOVÁ; Andrej BEŠŠE; Lenka RADOVÁ; Marek SVOBODA; Rostislav VYZULA a Ondřej SLABÝ. Identification and functional analysis of microRNA deregulated in colorectal cancer. In 3rd ICCTI Workshop - MicroRNAs in Oncology. 2012.

@proceedings{1076189,
   author = {Faltejsková, Petra and Mlčochová, Jitka and Šrůtová, Klára and Bešše, Andrej and Radová, Lenka and Svoboda, Marek and Vyzula, Rostislav and Slabý, Ondřej},
   booktitle = {3rd ICCTI Workshop - MicroRNAs in Oncology},
   keywords = {microRNAs; oncology; colorectal cancer; DLD-1; HCT-116},
   language = {eng},
   title = {Identification and functional analysis of microRNA deregulated in colorectal cancer},
   year = {2012}
}

TY  - CONF
ID  - 1076189
AU  - Faltejsková, Petra - Mlčochová, Jitka - Šrůtová, Klára - Bešše, Andrej - Radová, Lenka - Svoboda, Marek - Vyzula, Rostislav - Slabý, Ondřej
PY  - 2012
TI  - Identification and functional analysis of microRNA deregulated in colorectal cancer
KW  - microRNAs
KW  - oncology
KW  - colorectal cancer
KW  - DLD-1
KW  - HCT-116
N2  - Colorectal cancer (CRC) is one of the most common types of cancers in the Czech Republic and it is the third leading cause of cancer-related death in the Western countries. The main reasons of high mortality are diagnosis at the stage of advanced disease, insufficient clinical classification and low level of therapy individualization associated with unsatisfactory therapy responses. That is the reason why several efforts have been made to find new biomarkers in CRC enabling early diagnosis of asymptomatic disease, accurate differentiation between particular clinical stages and individualization of the therapy. MicroRNAs (miRNAs) are small, non-coding, single-stranded RNAs, 18 25 nucleotides in length, that are endogenously expressed and post-transcriptionally regulate gene expression. There is an increasing evidence that miRNAs can function as both tumor suppressors and as oncogenes and therefore play an important role in regulation of many biological processes such as cell cycle, proliferation, differentiation, apoptosis or invasiveness. The aim of our study was to determine the expression profiles of 667 miRNAs in CRC tissues and paired non-tumoral tissues and identify miRNAs with deregulated expression in CRC. Selected miRNAs were further validated and their role in regulation of cell cycle, apoptosis, cell viability and metastasing was analyzed in vitro using transient transfection and stable cell lines derived from colorectal cancer. We suppose that miRNAs or proteins that are under their control could serve as potential diagnostic, prognostic and predictive biomarkers, which can be identified not only in tumor tissues, but also in serum of CRC patients. The results of our research will be presented.
ER  -

FALTEJSKOVÁ, Petra; Jitka MLČOCHOVÁ; Klára ŠRŮTOVÁ; Andrej BEŠŠE; Lenka RADOVÁ; Marek SVOBODA; Rostislav VYZULA a Ondřej SLABÝ. Identification and functional analysis of microRNA deregulated in colorectal cancer. In \textit{3rd ICCTI Workshop - MicroRNAs in Oncology}. 2012.

Přehled o publikaci